Diffusion weighted imaging and magnetic resonance spectroscopy in a low flow ischaemia model due to endothelin induced vasospasm
- 1 May 2000
- journal article
- research article
- Published by Wiley in NMR in Biomedicine
- Vol. 13 (3), 154-162
- https://doi.org/10.1002/1099-1492(200005)13:3<154::aid-nbm620>3.0.co;2-w
Abstract
The aim of this MR study was to determine if vasospasm induced by application of endothelin-1 (ET-1) in the rat brain would model the abnormalities attributed to vasospasm described in patients with subarachnoid haemorrhage (SAH) with reversible neurological deficits. Following application of ET-1 in concentrations of 10(-4) M or 10(-6) M to the middle cerebral artery, there was an immediate drop in pH, an increase in the inorganic phosphate (P(i)) to phosphocreatine (PCr) ratio and elevated lactate. There was gradual recovery to control in the 10(-6) M group, but in the 10(-4) M group there was a loss of approximately 10% in the absolute signal intensities of PCr and adenosine triphosphate (ATP). In a second similarly treated group of animals, the area of the hemisphere with a low apparent diffusion coefficient (ADC) was 27 +/- 6% at 30 min and remained at about 20-21% at 90 min and beyond. Together these data suggest that the regions with persistently low ADC were metabolically compromised, with incomplete recovery of PCr and ATP, and represent irreversibly damaged tissue. This raises the possibility that MR spectroscopy and imaging could be a sensitive indicator of tissue viability. This is a potentially useful model of low flow as seen in clinical vasospasm following SAH.Keywords
This publication has 33 references indexed in Scilit:
- Diffusion MR Imaging During Acute Subarachnoid Hemorrhage in RatsStroke, 1998
- Topographic profile of reperfusion into MCA territory following endothelin-1-induced transient focal cerebral ischaemiaNeuroscience Letters, 1996
- Cerebral arterial spasm-a clinical reviewBritish Journal Of Neurosurgery, 1995
- Phosphorus-31 magnetic resonance spectra revealprolonged intracellular acidosis in the brain following subarachnoidhemorrhage.Proceedings of the National Academy of Sciences of the United States of America, 1994
- Inhibition of nitric oxide synthesis does not reduce infarct volume in a rat model of focal cerebral ischaemiaNeuroscience Letters, 1992
- Animal Models of Cerebral Ischemia. 2. Rat ModelsCerebrovascular Diseases, 1991
- Animal Models of Cerebral Ischemia. 1. Review of LiteratureCerebrovascular Diseases, 1991
- The International Cooperative Studyon the Timing of Aneurysm SurgeryJournal of Neurosurgery, 1990
- Rodent models of cerebral ischemia.Stroke, 1989
- Focal Cerebral Ischaemia in the Rat: 1. Description of Technique and Early Neuropathological Consequences following Middle Cerebral Artery OcclusionJournal of Cerebral Blood Flow & Metabolism, 1981