Biosynthetic Pathways of Inositol and Glycerol Phosphodiesters Used by the HyperthermophileArchaeoglobus fulgidusin Stress Adaptation
Open Access
- 1 December 2006
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 188 (23), 8128-8135
- https://doi.org/10.1128/jb.01129-06
Abstract
Archaeoglobus fulgidus accumulates di-myo-inositol phosphate (DIP) and diglycerol phosphate (DGP) in response to heat and osmotic stresses, respectively, and the level of glycero-phospho-myo-inositol (GPI) increases primarily when the two stresses are combined. In this work, the pathways for the biosynthesis of these three compatible solutes were established based on the detection of the relevant enzymatic activities and characterization of the intermediate metabolites by nuclear magnetic resonance analysis. The synthesis of DIP proceeds from glucose-6-phosphate via four steps: (i) glucose-6-phosphate was converted into l-myo-inositol 1-phosphate by l-myo-inositol 1-phosphate synthase; (ii) l-myo-inositol 1-phosphate was activated to CDP-inositol at the expense of CTP; this is the first demonstration of CDP-inositol synthesis in a biological system; (iii) CDP-inositol was coupled with l-myo-inositol 1-phosphate to yield a phosphorylated intermediate, 1,1′-di-myo-inosityl phosphate 3-phosphate (DIPP); (iv) finally, DIPP was dephosphorylated into DIP by the action of a phosphatase. The synthesis of the two other polyol-phosphodiesters, DGP and GPI, proceeds via the condensation of CDP-glycerol with the respective phosphorylated polyol, glycerol 3-phosphate for DGP and l-myo-inositol 1-phosphate for GPI, yielding the respective phosphorylated intermediates, 1X,1′X-diglyceryl phosphate 3-phosphate (DGPP) and 1-(1X-glyceryl) myo-inosityl phosphate 3-phosphate (GPIP), which are subsequently dephosphorylated to form the final products. The results disclosed here represent an important step toward the elucidation of the regulatory mechanisms underlying the differential accumulation of these compounds in response to heat and osmotic stresses.Keywords
This publication has 46 references indexed in Scilit:
- Occurrence of 1-Glyceryl-1-myo-Inosityl Phosphate in HyperthermophilesApplied and Environmental Microbiology, 2006
- Characterization of the Biosynthetic Pathway of Glucosylglycerate in the Archaeon Methanococcoides burtoniiJournal of Bacteriology, 2006
- Purified, Recombinant TagF Protein from Bacillus subtilis 168 Catalyzes the Polymerization of Glycerol Phosphate onto a Membrane Acceptor in VitroOnline Journal of Public Health Informatics, 2003
- A variant of the hyperthermophileArchaeoglobus fulgidusadapted to grow at high salinityFEMS Microbiology Letters, 2003
- The biosynthesis pathway of di-myo-inositol-1,1′-phosphate in Pyrococcus woeseiFEMS Microbiology Letters, 1998
- Synthesis of LL‐di‐myo‐inositol‐1,1′‐phosphate: A novel inositol phosphate from Pyrococcus woeseiRecueil des Travaux Chimiques des Pays-Bas, 1994
- Di‐myo‐inositol‐1, 1′‐phosphate: A new inositol phosphate isolated from Pyrococcus woeseiFEBS Letters, 1992
- The synthesis of DL‐1‐(hexadecanoyloxy)methyl‐ and 1‐O‐hexadecanoyl‐inositols as potential inhibitors of phospholipase CHelvetica Chimica Acta, 1990
- Nucleoside Polyphosphates. XI.1 An Improved General Method for the Synthesis of Nucleotide Coenzymes. Syntheses of Uridine-5', Cytidine-5' and Guanosine-5' Diphosphate DerivativesJournal of the American Chemical Society, 1961
- Nucleoside Polyphosphates. X.1 The Synthesis and Some Reactions of Nucleoside-5' Phosphoromorpholidates and Related Compounds. Improved Methods for the Preparation of Nucleoside-5' Polyphosphates1Journal of the American Chemical Society, 1961