Ultrasensitive fluorescence polarization DNA detection by target assisted exonuclease III-catalyzed signal amplification
- 8 February 2011
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Chemical Communications
- Vol. 47 (12), 3478-3480
- https://doi.org/10.1039/c0cc05703g
Abstract
Single stranded DNA sequences can be detected by target assisted exonuclease III-catalyzed signal amplification fluorescence polarization (TAECA-FP). The method offers an impressive detection limit of 83 aM within one hour for DNA detection and exhibits high discrimination ability even against a single base mismatch.Keywords
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