Changes of cytokine levels during granulocyte-colony-stimulating factor stem cell mobilization in healthy donors: association with mobilization efficiency and potential predictive significance
- 30 August 2010
- journal article
- Published by Wiley in Transfusion
- Vol. 51 (2), 319-327
- https://doi.org/10.1111/j.1537-2995.2010.02863.x
Abstract
Peripheral blood stem cells are an important source of hematopoietic stem cells (HSCs) for allogeneic transplantations. Some allogeneic donors mobilize HSCs poorly in response to the granulocyte--colony-stimulating factor (G-CSF). The estimation of the mobilization result in an individual donor is difficult due to the absence of suitable predictive factors. We analyzed the concentrations and kinetics of certain cytokines induced by G-CSF in 76 healthy donors and compared them with the mobilization efficiency. The levels of the most cytokines increased after the G-CSF application: sICAM, sVCAM, MMP-9, interleukin (IL)-6, TNF-α, sE-selectin, and fibronectin. The concentrations of SDF-1α and IL-8 decreased and VEGF and fractalkine remained unchanged. The premobilization concentrations of IL-6 (p = 0.0093) and TNF-a (p = 0.0006) correlated with preapheresis CD34+ cell count. The comparison of premobilization cytokine levels between better and worse mobilizers showed a difference for TNF-α (p = 0.0006) and IL-6 (p = 0.0682). The TNF-α level below cutoff of 3.6 pg/mL implied approximately 20 times higher risk of poor mobilization (odds ratio, 19.9; p = 0.0002). The immunophenotyping of CD34+ cells suggested a negative correlation between Day +5 CD34+ count and expression of CD11a (p = 0.0319) and a positive correlation with CD44 antigen expression (p = 0.0096). The concentrations of certain cytokines corresponded to the quality of HSC mobilization in healthy donors. Their levels measured before mobilization could probably serve as predictive factors for mobilization efficacy and prospectively detect donors who might profit from new mobilization molecules.Keywords
This publication has 29 references indexed in Scilit:
- Stem Cell Migration: A Quintessential Stepping Stone to Successful TherapyCurrent Stem Cell Research & Therapy, 2007
- Mechanisms of hematopoietic stem cell mobilization: When innate immunity assails the cells that make blood and boneExperimental Hematology, 2006
- CD44 and hyaluronic acid cooperate with SDF-1 in the trafficking of human CD34+ stem/progenitor cells to bone marrowPublished by American Society of Hematology ,2004
- Interleukin-6 is a major effector molecule of short-term G-CSF treatment inducing bone metabolism and an acute-phase responseExperimental Hematology, 2001
- Mobilization Kinetics of CD34 + Cells in Association with Modulation of CD44 and CD31 Expression during Continuous Intravenous Administration of G‐CSF in Normal DonorsThe International Journal of Cell Cloning, 2000
- Mobilization of hematopoietic primitive and committed progenitor cells into blood in mice by anti-vascular adhesion molecule-1 antibody alone or in combination with granulocyte colony-stimulating factorExperimental Hematology, 2000
- Expression of the Adhesion Molecules CD49d and CD49e on G-CSF-Mobilized CD34+Cells of Patients with Solid Tumors or Non-Hodgkin's and Hodgkin's Lymphoma and of Healthy Donors Is Inversely Correlated with the Amount of Mobilized CD34+CellsJournal of Hematotherapy & Stem Cell Research, 1999
- Endogenous Interleukin-8 (IL-8) Surge in Granulocyte Colony-Stimulating Factor–Induced Peripheral Blood Stem Cell MobilizationBlood, 1999
- Role of Adhesion Molecules in the Homing and Mobilization of Murine Hematopoietic Stem and Progenitor CellsBlood, 1998
- Mobilization and homing of peripheral blood progenitors is related to reversible downregulation of alpha4 beta1 integrin expression and function.JCI Insight, 1998