The dynamics of brain and cerebrospinal fluid growth in normal versus hydrocephalic mice
- 1 July 2010
- journal article
- Published by Journal of Neurosurgery Publishing Group (JNSPG) in Journal of Neurosurgery: Pediatrics
- Vol. 6 (1), 1-10
- https://doi.org/10.3171/2010.4.peds1014
Abstract
Hydrocephalus has traditionally been quantified by linear measures of ventricular size, with adjunct use of cortical mantle thickness. However, clinical outcome depends on cognitive function, which is more directly related to brain volume than these previous measures. The authors sought to quantify the dynamics of brain and ventricular volume growth in normal compared with hydrocephalic mice. Hydrocephalus was induced in 14-day-old C57BL/6 mice by percutaneous injection of kaolin into the cisterna magna. Nine hydrocephalic and 6 normal mice were serially imaged from age 2-12 weeks with a 14.1-T MR imaging unit. Total brain and ventricle volumes were calculated, and linear discriminant analysis was applied. Two very different patterns of response were seen in hydrocephalic mice compared with mice with normative growth. In one pattern (3 mice) brain growth was normal despite accumulation of CSF, and in the second pattern (6 mice) abnormal brain enlargement was accompanied by increased CSF volume along with parenchymal edema. In this latter pattern, spontaneous ventricular rupture led to normalization of brain volume, implying edema from transmantle pressure gradients. These 2 patterns of hydrocephalus were significantly discriminable using linear discriminant analysis (p < 0.01). In contrast, clinically relevant measurements of head circumference or frontal and occipital horn ratios were unable to discriminate between these patterns. This study is, to the authors' knowledge, the first serial quantification of the growth of brain and ventricle volumes in normal versus hydrocephalic development. The authors' findings demonstrate the feasibility of constructing normative curves of brain and fluid growth as complements to normative head circumference curves. By measuring brain volumes, distinct patterns of brain growth and enlargement can be observed, which are more likely linked to cognitive development and clinical outcome than fluid volumes alone.Keywords
This publication has 27 references indexed in Scilit:
- Characterization of juvenile and young adult mice following induction of hydrocephalus with kaolinExperimental Neurology, 2009
- Future Directions for Therapy of Childhood Hydrocephalus: A View from the LaboratoryPediatric Neurosurgery, 2001
- Recommendations for Preventive Pediatric Health CarePediatrics, 2000
- Magnetic Resonance Imaging and Behavioral Analysis of Immature Rats with Kaolin-Induced Hydrocephalus: Pre- and Postshunting ObservationsExperimental Neurology, 1997
- Ultrastructural Changes in the Deep Cortical Pyramidal Cells of Infant Rats with Inherited Hydrocephalus and the Effect of Shunt TreatmentExperimental Neurology, 1997
- An Introduction to the BootstrapPublished by Springer Science and Business Media LLC ,1993
- Quantitative nuclear magnetic resonance imaging: characterisation of experimental cerebral oedema.Journal of Neurology, Neurosurgery & Psychiatry, 1987
- Nuclear magnetic resonance imaging and spectroscopy in experimental brain edema in a rat modelJournal of Neurosurgery, 1986
- CONGENITAL CEREBRAL CYSTS OF THE CAVUM SEPTI PELLUCIDI (FIFTH VENTRICLE) AND CAVUM VERGAE (SIXTH VENTRICLE)Archives of Neurology & Psychiatry, 1931
- EXPERIMENTAL HYDROCEPHALUSAnnals of Surgery, 1919