Optimal patch application time in the evaluation of skin irritation
- 18 February 2013
- journal article
- research article
- Published by Wiley in The Journal of Dermatology
- Vol. 40 (5), 363-369
- https://doi.org/10.1111/1346-8138.12004
Abstract
We investigated the optimum application for evaluating skin irritation response by using samples of irritants commonly used as additives in cosmetics and other common household products. We studied 47 volunteers (16 men and 31 women). We selected three types of surfactant, one moisturizer, one anti-infective agent and one oil solution. Using Finn chambers on Scanpor tape, we performed the patch test. A total of 0.015 mL of each sample was applied to the Finn chamber. For liquids, circular filter paper was soaked in 0.015 mL of the sample. Samples were placed on the upper back of participants, and closed for 4, 24 or 48 h. A patch application time of 24 h is sufficient to detect primary skin irritation from irritants in cosmetics and other common household products. In addition, we found that skin irritation reactions were strongest at 24 h after patch removal and that the reaction tended to be weaker at 48 h after patch removal. Patch testing to evaluate irritants should be performed by means of a 24-h patch test with a follow-up reading at 24 h after patch removal. An application time of 24 h places less of a burden on patients than a 48-h patch test.Keywords
This publication has 14 references indexed in Scilit:
- Quantitative evaluation of patch test reactions: a comparison between visual grading and erythema index image analysisSkin Research and Technology, 2011
- Determination of skin irritation potential in the human 4‐h patch testContact Dermatitis, 2004
- Evaluation of modifications of the traditional patch test in assessing the chemical irritation potential of feminine hygiene productsSkin Research and Technology, 2004
- Multicentre study for the development of an in vivo model to evaluate the influence of topical formulations on irritationContact Dermatitis, 2000
- Patch tests versus use tests in skin irritation risk assessmentContact Dermatitis, 1998
- Clinical morphology of sodium lauryl sulfate (SLS) and nonanoic acid (NAA) irritant patch test reactions at 48 h and 96 h in 152 subjectsContact Dermatitis, 1998
- Interlaboratory evaluation of a human patch test for the identification of skin irritation potential/hazardFood and Chemical Toxicology, 1997
- Assessment of skin irritancy by 2 short tests compared to acute irritation induced by sodium lauryl sulfateContact Dermatitis, 1995
- Racial differences in sodium lauryl sulphate induced cutaneous irritation: black and whiteContact Dermatitis, 1988
- Irritant reactivity in males and femalesContact Dermatitis, 1987