The gene encoding the stem cell antigen, CD34, is conserved in mouse and expressed in haemopoietic progenitor cell lines, brain, and embryonic fibroblasts

Abstract

The human haemopoietic cell surface antigen, CD34, is a 105–120 kd cell surface glycoprotein whose stage-specificexpression by stem cells and lineage-specific progenitor cells suggests a role in regulating early events In blood cell differentiation. A murine gene and cDNA encoding a closely homologous protein have been isolated. The gene is organized In eight exons In 22 kb of DNA. The first exon lies in a GC- and CpG-rich island. The sequence of the gene and the cDNA predict a 382 amino acid-long protein containing an N-termlnal signal peptide and one transmembrane region 73 amino acids from the C-terminus. The extracellular part of the protein contains: a 140 amino acid-long-N-terminal region, 40% of whose residues are serine or threonine potential attachment sites for O-linked carbohydrate, as well as five potential attachment sites for N-llnked carbohydrate. Proximal to the extracellular membrane there is a 79 amino acid-long cystelne-rich region. The homology with the human sequence Is highest In the Intracellular domain (90% amino acid Identity) and lowest In the N-terminal region (43% amino acid identity). The protein is not homologous with any other proteins currently in the databases. The expression of the murine gene by a number of haemopoietic progenitor cell lines suggests that the CD34 function in haemopoiesis may be conserved between man and mouse. The high level of expression In a number of embryonic fibroblast cell lines and In brain Imply a function outside of haemopoiesis.