Diffuse reflectance spectroscopy: diagnostic accuracy of a non-invasive screening technique for early detection of malignant changes in the oral cavity
Open Access
- 24 June 2011
- Vol. 1 (1), e000071
- https://doi.org/10.1136/bmjopen-2011-000071
Abstract
Background Strong proof-of-principle for utilisation of diffuse reflectance spectroscopy, a non-invasive tool for early detection of malignant changes, has emerged recently. The potential of this technique in distinguishing normal tissue from hyperplastic and dysplastic tissues was explored. Methods Diffuse reflectance (DR) spectra in the 400–700 nm region were obtained from the buccal mucosa of 96 patients and 34 healthy volunteers. The DR spectral data were compared against the gold standard biopsy and histopathology results. A principal-component analysis was performed for dimensional reduction in the normalised spectral data with linear discriminant analysis as the classifying technique. The receiver operator characteristic curve technique was employed for evaluating the performance of the diagnostic test. Results DR spectral features for different lesions, such as normal/healthy, hyperplastic, dysplastic and squamous cell carcinoma (SCC), varied significantly according to the intensity of oxygenated haemoglobin absorption. While the classification based on discriminant scores provided an overall sensitivity of 98.5% and specificity of 96.0% for distinguishing SCC from dysplasia, they were 100.0% and 95.0%, respectively, for distinguishing dysplasia from hyperplasia. Similarly, the analysis yielded a sensitivity of 95.0% and specificity of 100.0% for distinguishing hyperplasia from healthy tissue. The areas under the receiver operator characteristic curves were 0.98 (95% CI 0.95 to 1.00) and 0.95 (95% CI 0.90 to 1.00) for distinguishing dysplasia from SCC and hyperplasia from dysplasia, respectively. Conclusion DR spectral data efficiently discriminate healthy tissue from oral malignant lesions. Diagnostic accuracies obtained in this study highlight the potential use of this method for routine clinical practice.This publication has 22 references indexed in Scilit:
- Prospective evaluation of a portable depth-sensitive optical spectroscopy device to identify oral neoplasiaBiomedical Optics Express, 2010
- Discriminant analysis of autofluorescence spectra for classification of oral lesions in vivoLasers in Surgery and Medicine, 2009
- Oral squamous cell carcinoma overviewOral Oncology, 2009
- Noninvasive evaluation of oral lesions using depth‐sensitive optical spectroscopyCancer, 2009
- Oxygenated hemoglobin diffuse reflectance ratio for in vivo detection of oral pre-cancerJournal of Biomedical Optics, 2008
- Natural history of potentially malignant oral lesions and conditions: an overview of the literatureJournal of Oral Pathology & Medicine, 2007
- Comparison of a physical model and principal component analysis for the diagnosis of epithelial neoplasias in vivo using diffuse reflectance spectroscopyOptics Express, 2007
- Oral cancer detection using diffuse reflectance spectral ratio R540∕R575 of oxygenated hemoglobin bandsJournal of Biomedical Optics, 2006
- Spectroscopic diagnosis and imaging of invisible pre-cancerFaraday Discussions, 2003
- Trimodal spectroscopy for the detection and characterization of cervical precancers in vivoAmerican Journal of Obstetrics and Gynecology, 2002