Protein tyrosine nitration in pea roots during development and senescence
Top Cited Papers
Open Access
- 23 February 2013
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 64 (4), 1121-1134
- https://doi.org/10.1093/jxb/ert006
Abstract
Protein tyrosine nitration is a post-translational modification mediated by reactive nitrogen species (RNS) that is associated with nitro-oxidative damage. No information about this process is available in relation to higher plants during development and senescence. Using pea plants at different developmental stages (ranging from 8 to 71 days), tyrosine nitration in the main organs (roots, stems, leaves, flowers, and fruits) was analysed using immunological and proteomic approaches. In the roots of 71-day-old senescent plants, nitroproteome analysis enabled the identification a total of 16 nitrotyrosine-immunopositive proteins. Among the proteins identified, NADP-isocitrate dehydrogenase (ICDH), an enzyme involved in the carbon and nitrogen metabolism, redox regulation, and responses to oxidative stress, was selected to evaluate the effect of nitration. NADP-ICDH activity fell by 75% during senescence. Analysis showed that peroxynitrite inhibits recombinant cytosolic NADP-ICDH activity through a process of nitration. Of the 12 tyrosines present in this enzyme, mass spectrometric analysis of nitrated recombinant cytosolic NADP-ICDH enabled this study to identify the Tyr392 as exclusively nitrated by peroxynitrite. The data as a whole reveal that protein tyrosine nitration is a nitric oxide-derived PTM prevalent throughout root development and intensifies during senescence.Keywords
This publication has 77 references indexed in Scilit:
- NADP-Dependent Isocitrate Dehydrogenase fromArabidopsisRoots Contributes in the Mechanism of Defence against the Nitro-Oxidative Stress Induced by SalinityThe Scientific World Journal, 2012
- Glutamine Synthetase Is a Molecular Target of Nitric Oxide in Root Nodules of Medicago truncatula and Is Regulated by Tyrosine NitrationPlant Physiology, 2011
- The exudate from an arbuscular mycorrhizal fungus induces nitric oxide accumulation in Medicago truncatula rootsMycorrhiza, 2011
- In vivo protein tyrosine nitration in Arabidopsis thalianaJournal of Experimental Botany, 2011
- Inhibition of Arabidopsis O-Acetylserine(thiol)lyase A1 by Tyrosine NitrationPublished by Elsevier BV ,2011
- Draft genome sequence of the oilseed species Ricinus communisNature Biotechnology, 2010
- Peroxisomes Are Required for in Vivo Nitric Oxide Accumulation in the Cytosol following Salinity Stress of Arabidopsis PlantsPlant Physiology, 2009
- Nitric oxide: an active nitrogen molecule that modulates cellulose synthesis in tomato rootsNew Phytologist, 2008
- Nitric oxide plays a central role in determining lateral root development in tomatoPlanta, 2004
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970