MicroRNA-141 Regulates Smad Interacting Protein 1 (SIP1) and Inhibits Migration and Invasion of Colorectal Cancer Cells
- 15 October 2009
- journal article
- Published by Springer Science and Business Media LLC in Digestive Diseases and Sciences
- Vol. 55 (8), 2365-2372
- https://doi.org/10.1007/s10620-009-1008-9
Abstract
Background Colorectal cancer (CRC) is the third most common cancer in the world. Despite recent advances in diagnostics and treatment, prognosis for patients with advanced disease is still poor. microRNAs (miRNAs) are a class of endogenous, small noncoding RNA molecules which are crucial regulators of gene expression at the posttranscriptional level. miRNAs participate in many biological events and play an important role in various human diseases, including CRC. Aims This study is to identify the role of miR-141 in migration and invasion of CRC cells. Methods Expression of miR-141 and Smad interacting protein 1 (SIP1) were detected by real-time reverse-transcription polymerase chain reaction (RT-PCR) and Western blotting. The effect of miR-141 on migration and invasion of CRC cells was investigated using wound healing assay and Matrigel invasion assay in vitro. The regulation effect of miR-141 on SIP1 was evaluated by dual-luciferase reporter assay. Results We demonstrated that miR-141 levels correlate inversely with SIP1 protein levels as well as cell migration and invasion of CRC cells. SIP1 was identified as a functional target of miR-141. Conclusions miR-141 regulates SIP1 to inhibit migration and invasion of CRC cells. miR-141 and SIP1 might be candidate therapeutic targets in CRC.Keywords
This publication has 38 references indexed in Scilit:
- miR-200 Regulates PDGF-D-Mediated Epithelial–Mesenchymal Transition, Adhesion, and Invasion of Prostate Cancer CellsThe International Journal of Cell Cloning, 2009
- Epigenetic Silencing of MicroRNA-34b/c and B-Cell Translocation Gene 4 Is Associated with CpG Island Methylation in Colorectal CancerCancer Research, 2008
- The miR-200 Family Inhibits Epithelial-Mesenchymal Transition and Cancer Cell Migration by Direct Targeting of E-cadherin Transcriptional Repressors ZEB1 and ZEB2Journal of Biological Chemistry, 2008
- A reciprocal repression between ZEB1 and members of the miR‐200 family promotes EMT and invasion in cancer cellsEMBO Reports, 2008
- MicroRNA Expression Profiles in Serous Ovarian CarcinomaClinical Cancer Research, 2008
- MicroRNA Expression Profiles Associated With Prognosis and Therapeutic Outcome in Colon AdenocarcinomaJAMA, 2008
- Altered Expression of miR-21, miR-31, miR-143 and miR-145 Is Related to Clinicopathologic Features of Colorectal CancerOncology, 2007
- SIP1/ZEB2 induces EMT by repressing genes of different epithelial cell-cell junctionsNucleic Acids Research, 2005
- Reduced accumulation of specific microRNAs in colorectal neoplasia.2003
- High-throughput retroviral tagging to identify components of specific signaling pathways in cancerNature Genetics, 2002