Demineralization of Hard Tissues by Organic Chelating Agents at Neutral pH
- 1 December 1953
- journal article
- research article
- Published by SAGE Publications in Journal of Dental Research
- Vol. 32 (6), 859-867
- https://doi.org/10.1177/00220345530320061401
Abstract
The Na salts of ethylene-diamine tetra acetic acid (Edt) form soluble non-ionic chelates with Ca and other metallic ions over a wide pH range. A 0.5 [image] soln. of the Na salt of Edt neutralized to a pH of 7.4 by HCl makes a suitable demineralizing media for small specimens of bone and tooth tissue. The solns. demineralize bone specimens at a rate about 14% that of acid solns. and about 7 times as rapidly as a neutral decalcifying soln. of Mg citrate. Higher temps. (60[degree] C.) increase the rate of demoralization. Tissue demineralized by Edt possesses excellent staining properties.Keywords
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