Spray-dried plasma improves growth performance and reduces inflammatory status of weaned pigs challenged with enterotoxigenic Escherichia coli K881
- 1 June 2004
- journal article
- Published by Oxford University Press (OUP) in Journal of Animal Science
- Vol. 82 (6), 1764-1772
- https://doi.org/10.2527/2004.8261764x
Abstract
We investigated whether spray-dried plasma (SDP) improved growth and health of piglets challenged with enterotoxigenic Escherichia coli K88 (ETEC). Forty-eight pigs weaned at 21 d (BW = 4.88 ± 0.43 kg) received one of four diets containing 6% SDP or fish proteins (as-fed basis) either nonmedicated (SDP-NM and FP-NM diets) or medicated with 0 or 250 mg/kg of colistine + 500 mg/kg of amoxycycline (SDP-M and FP-M diets), for 15 d. On d 4, pigs were orally challenged with ETEC. On d 15, eight pigs per dietary group were killed, blood and saliva were collected for analysis of K88 fimbriae-specific immunoglobulin (Ig)-A, and jejunum was removed for villi preparation, histological analysis, and cytokine expression. The presence or absence of K88 receptors (K88+ and K88− pigs respectively) was determined by villous adhesion assay. Effects of protein source on ADG (P = 0.04) and ADFI (P < 0.01), as well of medication on ADFI (P < 0.02), of all pigs were observed. In sacrified pigs, there was an effect of protein source on ADG (P = 0.03) and ADFI (P < 0.001), as well an interaction between medication and presence of K88 receptor (P = 0.02) for feed:gain ratio. Plasma K88 specific IgA were low in all K88− pigs and higher in K88+ pigs fed FP-NM compared with all the other groups (P < 0.05), except SDP-M. An interaction was found among protein source, medication, and presence of K88 receptors (P = 0.04). Saliva IgA concentrations were high in all pigs fed FP-NM and low in all other pigs. Jejunum of pigs fed FP-NM showed some ulcerations, edema, and mild inflammatory cell infiltration (ICI). In pigs fed FP-M, edema was reduced. Conversely, only a mild ICI was observed in pigs fed SDP-NM and SDP-M. Crypt depth was increased in K88+ pigs fed SDP-NM and an interaction between protein source and presence of K88 receptors was observed (P < 0.05). Expressions of tumor necrosis factor-α and interleukin (IL)-8 were lower in pigs fed SDP-NM and SDP-M than in those fed FP-NM and FP-M, either K88− or K88+ (P < 0.01). In pigs fed FP diets, expression of IL-8 tended to increase (P = 0.08) in K88+ compared with K88− subjects. Expression of interferon-γ increased in K88− and K88+ pigs fed FP-M as compared with other pigs (P < 0.01). These results indicate that feeding with SDP improved growth performance and protected against E. coli-induced inflammatory status, and suggest that use of SDP-NM can be considered a valid antibiotic alternative. Copyright © 2004. . Copyright 2004 Journal of Animal ScienceKeywords
This publication has 19 references indexed in Scilit:
- Response of early-weaned pigs to an enterotoxigenic Escherichia coli (K88) challenge when fed diets containing spray-dried porcine plasma or pea protein isolate plus egg yolk antibody1Journal of Animal Science, 2003
- Response of early-weaned pigs to an enterotoxigenic Escherichia coli (K88) challenge when fed diets containing spray-dried porcine plasma or pea protein isolate plus egg yolk antibody, zinc oxide, fumaric acid, or antibiotic1Journal of Animal Science, 2003
- Evaluation of Receptor Binding Specificity ofEscherichia coliK88 (F4) Fimbrial Adhesin Variants Using Porcine Serum Transferrin and Glycosphingolipids as Model ReceptorsInfection and Immunity, 2002
- Small Intestinal Morphology and Disaccharidase Activities in Early-weaned Piglets Fed a Diet Containing Spray-dried Porcine PlasmaJournal of Veterinary Medicine Series A, 2002
- Small intestinal morphology in weaned piglets fed a diet containing spray-dried porcine plasmaResearch in Veterinary Science, 2001
- Growth performance of weanling pigs fed spray-dried animal plasma: a reviewLivestock Production Science, 2001
- The distribution and stability of Escherichia coli K88 receptor in the gastrointestinal tract of the pigVeterinary Microbiology, 1994
- Inhibition of adhesion of enterotoxigenic Escherichia coli cells expressing F17 fimbriae to small intestinal mucus and brush-border membranes of young calvesMicrobial Pathogenesis, 1993
- Vaccines for preventing enterotoxigenic Escherichia coli infections in farm animalsVaccine, 1993
- Antimicrobial Agents Induce Monocytes to Release IL-1α, IL-6, and Tnf and Induce Lymphocytes to Release IL-4 and TNFτImmunopharmacology and Immunotoxicology, 1992