Real-time measurement of dopamine fluctuations after cocaine in the brain of behaving rats
- 8 July 2005
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 102 (29), 10023-10028
- https://doi.org/10.1073/pnas.0504657102
Abstract
Dopamine neurotransmission has been implicated in the modulation of many cognitive processes. Both rapid (phasic) and slower (tonic) changes in its extracellular concentration contribute to its complex actions. Fast in vivo electrochemical techniques can measure extracellular dopamine on a rapid time scale but without the selectivity afforded with slower techniques that use chemical separations. Cyclic voltammetry improves chemical resolution over other electrochemical methods, and it can resolve dopamine changes in the brains of behaving rodents over short epochs (<10 s). With this method, however, selective detection of slower dopamine changes is still elusive. Here we demonstrate that principal component regression of cyclic voltammetry data enables quantification of changes in dopamine and extracellular pH. Using this method, we show that cocaine modifies dopamine release in two ways: dopamine concentration transients increase in frequency and magnitude, whereas a gradual increase in steady-state dopamine concentration occurs over 90 s.Keywords
This publication has 49 references indexed in Scilit:
- The Effects of Vesicular Volume on Secretion through the Fusion Pore in Exocytotic Release from PC12 CellsJournal of Neuroscience, 2004
- Overoxidation of carbon-fiber microelectrodes enhances dopamine adsorption and increases sensitivityElectronic supplementary information (ESI) available: National Instruments Data Acquisition System. See http://www.rsc.org/suppdata/an/b3/b307024g/The Analyst, 2003
- Microdialysis of dopamine interpreted with quantitative model incorporating probe implantation traumaJournal of Neurochemistry, 2003
- Subsecond dopamine release promotes cocaine seekingNature, 2003
- ChemometricsAnalytical Chemistry, 2002
- Serotonin Transporter Function in vivo: Assessment by ChronoamperometryaAnnals of the New York Academy of Sciences, 1998
- In Vivo Electrochemical Studies of Dopamine Overflow and Clearance in the Striatum of Normal and MPTP‐Treated Rhesus MonkeysJournal of Neurochemistry, 1996
- Voltammetric and microdialysis monitoring of brain monoamine neurotransmitter release during sociosexual interactionsBehavioural Brain Research, 1995
- Control Procedures for Residuals Associated With Principal Component AnalysisTechnometrics, 1979