The transcriptional activation region of Msn2p, in Saccharomyces cerevisiae, is regulated by stress but is insensitive to the cAMP signalling pathway
- 18 February 2006
- journal article
- Published by Springer Science and Business Media LLC in Molecular Genetics and Genomics
- Vol. 275 (3), 277-287
- https://doi.org/10.1007/s00438-005-0017-4
Abstract
Msn2p is a transcription factor that mediates a transient cellular response to multiple stresses and to changes in the nutritional environment. It was previously shown that the C-terminal half of Msn2p contains the DNA binding domain, a nuclear localization signal and nuclear export determinants which are activated by stress. In this report, we demonstrate that the N-terminal half of Msn2p contains the transcriptional activation domain(s). In addition, we present evidence that this region of Msn2p is able to mediate both the activation of transcription and export of the protein from the nucleus in response to stress. Interestingly, while the stress response integrated by the components of the C-terminal half that are involved in nucleocytoplasmic localization is reversed by elevated levels of cAMP, the effects of stress on the transcriptional activation domain and the localization determinants present in the N-terminal half of Msn2p are insensitive to variations in the intracellular cAMP concentrations.Keywords
This publication has 31 references indexed in Scilit:
- Genetic Factors That Regulate the Attenuation of the General Stress Response of YeastGenetics, 2005
- Heat shock-induced degradation of Msn2p, a Saccharomyces cerevisiae transcription factor, occurs in the nucleusMolecular Genetics and Genomics, 2004
- Oscillatory nucleocytoplasmic shuttling of the general stress response transcriptional activators Msn2 and Msn4 in Saccharomyces cerevisiae The Journal of cell biology, 2003
- Yeast Glycogen Synthase Kinase-3 Activates Msn2p-dependent Transcription of Stress Responsive GenesMolecular Biology of the Cell, 2003
- The control of the yeast H2O2 response by the Msn2/4 transcription factorsMolecular Microbiology, 2002
- Negative regulation of Gcn4 and Msn2 transcription factors by Srb10 cyclin-dependent kinaseGenes & Development, 2001
- Stress-controlled transcription factors, stress-induced genes and stress tolerance in budding yeastFEMS Microbiology Reviews, 2000
- The heat shock response in yeast: differential regulations and contributions of the Msn2p/Msn4p and Hsf1p regulonsMolecular Microbiology, 1999
- SDC25, a dispensable Ras guanine nucleotide exchange factor of Saccharomyces cerevisiae differs from CDC25 by its regulation.Molecular Biology of the Cell, 1996
- The pde2 gene of Saccharomyces cerevisiae is allelic to rcal and encodes a phosphodiesterase which protects the cell from extracellullar cAMPFEBS Letters, 1993