Angiotensin II Stimulates Endothelial NO Synthase Phosphorylation in Thoracic Aorta of Mice With Abdominal Aortic Banding Via Type 2 Receptor
Open Access
- 1 November 2006
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Hypertension
- Vol. 48 (5), 958-964
- https://doi.org/10.1161/01.hyp.0000244108.30909.27
Abstract
Abdominal aortic banding in mice induces upregulation of angiotensin II (Ang II) type 2 (AT 2 ) receptors in the pressure-overloaded thoracic aorta. To clarify mechanisms underlying the vascular AT 2 receptor-dependent NO production, we measured aortic levels of endothelial NO synthase (eNOS), eNOS phosphorylated at Ser 633 and Ser 1177 , protein kinase B (Akt), and Akt phosphorylated at Ser 473 in thoracic aortas of mice after banding. Total eNOS, both forms of phosphorylated eNOS, Akt, and phosphorylated Akt levels, as well as cGMP contents, were significantly increased 4 days after banding. The administration of PD123319 (an AT 2 receptor antagonist) or icatibant (a bradykinin B 2 receptor antagonist) abolished the banding-induced upregulation of both forms of phosphorylated eNOS, as well as elevation of cGMP, but did not affect the upregulation of eNOS, Akt, and phosphorylated Akt. In the in vitro experiments using aortic rings prepared from banded mice, Ang II produced significant increases in both forms of phosphorylated eNOS, as well as cGMP, and these effects were blocked by PD123319 and icatibant. Ang II–induced eNOS phosphorylation and cGMP elevation in aortic rings were inhibited by protein kinase A (PKA) inhibitors H89 and KT5720 but not by phosphatidylinositol 3-kinase inhibitors wortmannin and LY24002. The contractile response to Ang II was attenuated in aortic rings from banded mice via AT 2 receptor, and this attenuation was blocked by PKA inhibitors. These results suggest that the activation of AT 2 receptor by Ang II induces phosphorylation of eNOS at Ser 633 and Ser 1177 via a PKA-mediated signaling pathway, resulting in sustained activation of eNOS.Keywords
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