Age-related oxidative protein damages in central nervous system of rats: modulatory role of grape seed extract
- 31 October 2005
- journal article
- Published by Wiley in International Journal of Developmental Neuroscience
- Vol. 23 (6), 501-507
- https://doi.org/10.1016/j.ijdevneu.2005.06.001
Abstract
Oxidative stress has been shown to play a major role in aging and in neurodegenerative disorders. Protein modification is one of the important consequences of oxidative stress. In the present study, we evaluated the role of grape seed extract on memory, reactive oxygen species production, protein carbonyls (PCO), and thiol status in discrete regions of central nervous system of young and aged rats. Male albino rats of Wistar strain were divided into four groups: Group I—control young rats, Group II—young rats treated with grape seed extract (100 mg/kg BW) for 30 days, Group III—aged control rats and Group IV—aged rats supplemented with grape seed extract (100 mg/kg BW) for 30 days. Memory loss was observed in the aged rats. Age associated increase in reactive oxygen species production and protein oxidation was observed in the spinal cord; cerebral cortex, striatum and the hippocampus regions of aged rats (Group III). The levels of total thiol, non-protein thiol, protein thiols were found to be significantly decreased in spinal cord and all the brain regions studied in aged rats when compared to young rats. Supplementation of aged rats with grape seed extract showed increased memory performance and declined reactive oxygen species production, decreased protein carbonyl levels and improved thiol levels. These findings demonstrated that grape seed extract enhanced the antioxidant status and decreased the incidence of free radical induced protein oxidation in aged rats thereby protecting the central nervous system from the reactive oxygen species.Keywords
This publication has 39 references indexed in Scilit:
- Protective Effects of Oligomers of Grape Seed Polyphenols Against β‐Amyloid‐Induced Oxidative Cell DeathAnnals of the New York Academy of Sciences, 2004
- Age-associated decreased activities of mitochondrial electron transport chain complexes in heart and skeletal muscle: role of l-carnitineChemico-Biological Interactions, 2004
- Nutritional approaches to combat oxidative stress in Alzheimer’s diseaseThe Journal of Nutritional Biochemistry, 2002
- Structural and Functional Changes in Proteins Induced by Free Radical‐mediated Oxidative Stress and Protective Action of the Antioxidants N‐tert‐Butyl‐α‐phenylnitrone and Vitamin EaAnnals of the New York Academy of Sciences, 1998
- Structure-antioxidant activity relationships of flavonoids and phenolic acidsFree Radical Biology & Medicine, 1996
- Age-related changes of oxygen free radical production in the rat brain slice after hypoxia: on-line measurement using enhanced chemiluminescenceBrain Research, 1995
- Oxidative stress and aging in the Mongolian gerbil (Meriones unguiculatus)Mechanisms of Ageing and Development, 1995
- Role of Free Radicals in Aging and DiseaseAnnals of the New York Academy of Sciences, 1992
- Age influence on oxidative events during brain ischemia/reperfusionArchives of Gerontology and Geriatrics, 1991
- Role of Oxidative Stress in Development of Complications in DiabetesDiabetes, 1991