Bacteriophages as indicators of faecal pollution and enteric virus removal
Open Access
- 17 March 2017
- journal article
- review article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 65 (1), 11-26
- https://doi.org/10.1111/lam.12736
Abstract
Bacteriophages are an attractive alternative to faecal indicator bacteria (FIB), particularly as surrogates of enteric virus fate and transport, due to their closer morphological and biological properties. Based on a review of published data, we summarize densities of coliphages (F+ and somatic), Bacteroides spp. and enterococci bacteriophages (phages) in individual human waste, raw wastewater, ambient fresh and marine waters and removal through wastewater treatment processes utilizing traditional treatments. We also provide comparisons with FIB and enteric viruses whenever possible. Lastly, we examine fate and transport characteristics in the aquatic environment and provide an overview of the environmental factors affecting their survival. In summary, concentrations of bacteriophages in various sources were consistently lower than FIB, but more reflective of infectious enteric virus levels. Overall, our investigation indicates that bacteriophages may be adequate viral surrogates, especially in built systems, such as wastewater treatment plants. Significance and Impact of the Study Bacteriophage are alternative fecal indicators that may be better surrogates for viral pathogens than fecal indicator bacteria (FIB). This report offers a summary of the existing literature concerning the utility of bacteriophage as indicators of viral presence (fecal sources and surface waters) and persistence (in built infrastructure and aquatic environments). Our findings indicate that bacteriophage levels in all matrices examined are consistently lower than FIB, but similar to viral pathogens. Furthermore, in built infrastructure (e.g. wastewater treatment systems) bacteriophage closely mimic viral pathogen persistence suggesting they may be adequate sentinels of enteric virus removal.Keywords
This publication has 150 references indexed in Scilit:
- Sunlight Inactivation of Human Viruses and Bacteriophages in Coastal Waters Containing Natural PhotosensitizersEnvironmental Science & Technology, 2013
- Microbial Quality of Tropical Inland Waters and Effects of Rainfall EventsApplied and Environmental Microbiology, 2012
- Concentration of Norovirus during Wastewater Treatment and Its Impact on Oyster ContaminationApplied and Environmental Microbiology, 2012
- Occurrence and Persistence of Bacterial Pathogens and Indicator Organisms in Beach Sand along the California CoastApplied and Environmental Microbiology, 2012
- Isolation of bacteriophage host strains of Bacteroides species suitable for tracking sources of animal faecal pollution in waterEnvironmental Microbiology, 2011
- Presence of Enteric Viruses in Source Waters for Drinking Water Production in the NetherlandsApplied and Environmental Microbiology, 2010
- Viral Multiplex Quantitative PCR Assays for Tracking Sources of Fecal ContaminationApplied and Environmental Microbiology, 2010
- Gene Mapping and Phylogenetic Analysis of the Complete Genome from 30 Single-Stranded RNA Male-Specific Coliphages (Family Leviviridae )Journal of Virology, 2009
- Integrated Analysis of Established and Novel Microbial and Chemical Methods for Microbial Source TrackingApplied and Environmental Microbiology, 2006
- Bacterial transport in laboratory columns and filters: Influence of ionic strength and pH on collision efficiencyWater Research, 1995