Detection of Genes Encoding Internalization-Associated Proteins in Streptococcus pyogenes Isolates from Patients with Invasive Diseases and Asymptomatic Carriers
- 1 April 2007
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 45 (4), 1284-1287
- https://doi.org/10.1128/jcm.02119-06
Abstract
A total of 161 Streptococcus pyogenes isolates from patients with invasive infections or from asymptomatic carriers were examined for genes ( prtF1 , prtF2 , and fba ) coding for fibronectin-binding proteins to evaluate their involvement in the pathogenesis of different streptococcal manifestations. We found no significant differences in the presence of these three genes between the two groups. Overall, the prtF2 gene was present in similar percentages among strains from both sources (61% versus 63%). Strains carrying the gene fba were slightly more common among those isolated from asymptomatic carriers (72.6% versus 65%). Also, the prtF1 gene was present in a higher, but not significant, percentage among strains from throat swabs than among isolates from invasive infections (75% versus 64.9%). However, this more detailed characterization of the genes encoding fibronectin-binding proteins allowed us to identify a strong association of genes of the erm class, coding for macrolide resistance, with prtF1 and prtF2 rather than with prtF1 alone. Since macrolide resistance was significantly associated with throat swab isolates, it may be hypothesized that proteins coded by prtF1 and prtF2 genes may be synergic in providing support for cell invasion and/or colonizing or persistence efficiency.Keywords
This publication has 32 references indexed in Scilit:
- Association of group A streptococcal emm types with virulence traits and macrolide-resistance genes is independent of the source of isolationJournal of Medical Microbiology, 2005
- Presence of fibronectin-binding protein gene prtF2 in invasive group A streptococci in tropical Australia is associated with increased internalisation efficiencyMicrobes and Infection, 2005
- Streptococcus pyogenes Fibronectin-binding Protein F2Journal of Biological Chemistry, 2004
- Molecular Characterization of a Novel Fibronectin-binding Protein of Streptococcus pyogenesStrains Isolated from Toxic Shock-like Syndrome PatientsPublished by Elsevier BV ,2002
- Streptococcus pyogenes prtFII, but not sfbI, sfbII or fbp54, is represented more frequently among invasive-disease isolates of tropical AustraliaEpidemiology and Infection, 2002
- Role Played by the Fibronectin‐Binding Protein SfbI (Protein F1) ofStreptococcus pyogenesin Bacterial Internalization by Epithelial CellsThe Journal of Infectious Diseases, 1999
- Comparison of adherence to and penetration of a human laryngeal epithelial cell line by group A streptococci of various M protein typesFEMS Immunology & Medical Microbiology, 1999
- Ability of clinical isolates of group A streptococci to adhere to and invade HEp-2 epithelial cellsJournal of Medical Microbiology, 1998
- Invasion and Survival ofStreptococcus pyogenesin Eukaryotic Cells Correlates with the Source of the Clinical IsolatesThe Journal of Infectious Diseases, 1998
- Protein F2, a novel fibronectin‐binding protein from Streptococcus pyogenes, possesses two binding domainsMolecular Microbiology, 1996