A xylanase produced by the rumen anaerobic protozoanPolyplastron multivesiculatumshows close sequence similarity to family 11 xylanases from Gram-positive bacteria
Open Access
- 1 December 1999
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 181 (1), 145-152
- https://doi.org/10.1111/j.1574-6968.1999.tb08837.x
Abstract
We report for the first time the cloning and characterisation of a protozoal enzyme involved in plant cell wall polysaccharide degradation. A cDNA library was constructed from the ruminal protozoan Polyplastron multivesiculatum and a stable clone expressing xylanase activity was isolated. The encoded enzyme belongs to the glycoside hydrolase family 11, and phylogenetic analysis indicates a closer relationship with catalytic domains from Gram-positive bacteria than the other fibrolytic eukaryotes from the rumen, the anaerobic fungi.Keywords
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