Hepatoprotective and Antioxidant Activities ofVernonia amygdalinaon Acetaminophen-Induced Hepatic Damage in Mice
- 1 December 2006
- journal article
- Published by Mary Ann Liebert Inc in Journal of Medicinal Food
- Vol. 9 (4), 524-530
- https://doi.org/10.1089/jmf.2006.9.524
Abstract
Vernonia amygdalina Del. (Family Compositae) is used in Nigerian folk medicine as a tonic and remedy against constipation, fever, high blood pressure, and many infectious diseases. We have evaluated the hepatoprotective and antioxidant effects of an aqueous extract of V. amygdalina leaves against acetaminophen-induced hepatotoxicity and oxidative stress in mice in vivo. Activities of liver marker enzymes in serum (glutamate-oxaloacetate transaminase, glutamate-pyruvate transaminase, lactate dehydrogenase, and alkaline phosphatase) and bilirubin levels were determined colorimetrically, while catalase activity, lipid peroxidation products, thiobarbituric acid-reactive substances (TBARS), iron, and total protein concentrations were measured in liver homogenate. Acetaminophen challenge (300 mg/kg, i.p) for 7 days caused significant (P < .01) increases in the levels of bilirubin, liver enzymes, TBARS, and iron, while catalase activity and total protein level were reduced significantly (P < .01). Preadministration of V. amygdalina resulted in a dose-dependent (50-100 mg/kg) reversal of acetaminophen-induced alterations of all the liver function parameters by 51.9-84.9%. Suppression of acetaminophen-induced lipid peroxidation and oxidative stress by the extract was also dose-dependent (50-100 mg/kg). The results of this study suggest that V. amygdalina elicits hepatoprotectivity through antioxidant activity on acetaminophen-induced hepatic damage in mice.Keywords
This publication has 40 references indexed in Scilit:
- The protective effects of Propolis on hepatic injury and its mechanismPhytotherapy Research, 2003
- Influence of Piper betle on Hepatic Marker Enzymes and Tissue Antioxidant Status in Ethanol-Treated Wistar RatsJournal of Medicinal Food, 2002
- HEPATOPROTECTIVE EFFECTS OF 18β-GLYCYRRHETINIC ACID ON CARBON TETRACHLORIDE-INDUCED LIVER INJURY: INHIBITION OF CYTOCHROME P450 2E1 EXPRESSIONPharmacological Research, 2002
- Sesquiterpenes with Hepatoprotective Activity from Cnidium monnieri on Tacrine-Induced Cytotoxicity in Hep G2 CellsPlanta Medica, 2002
- Alcohol exposure and paracetamol‐induced hepatotoxicityAddiction Biology, 2002
- Role of CYP2E1 in the Hepatotoxicity of AcetaminophenJournal of Biological Chemistry, 1996
- Antitumoral and Antimicrobial Activities of Bitter Sesquiterpene Lactones ofVernonia amygdalina, a Possible Medicinal Plant Used by Wild ChimpanzeesBioscience, Biotechnology, and Biochemistry, 1993
- Molecular Aspects of Paracetamol-Induced Hepatotoxicity and its Mechanism-Based PreventionDrug Metabolism Reviews, 1992
- Mechanism of Antihepatotoxic Activity of Glycyrrhizin, I: Effect on Free Radical Generation and Lipid PeroxidationPlanta Medica, 1984
- A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. Coli I. Purification and characterization of alkaline phosphataseBiochimica et Biophysica Acta, 1960