Effects of Mucin, Haemoglobin and Collagen on the Maximum Specific Growth Rate, Biomass and Hydrolytic Enzyme Production ofPorphyromonas gingivalisin Continuous Culture
Open Access
- 1 January 1991
- journal article
- research article
- Published by Informa UK Limited in Microbial Ecology in Health & Disease
- Vol. 4 (5), 311-318
- https://doi.org/10.3109/08910609109140281
Abstract
The growth of Porphyromonas gingivalis was studied in continuous culture with proteins (mucin, haemoglobin and collagen) as limiting substrates. The results show that hydrolytic enzymes are produced by the organism at a rate which is sufficient to support rapid growth (μ = 0.13–0.34/h) and high yield (0.14–0.29 g dry weight/1) of cells. The activities of four hydrolytic enzymes (trypsin-like protease, alkaline phosphatase, N-acetyl-β-glucosaminidase and glycylprolyl dipeptidase) varied with the substrates provided. Only the trypsin-like protease appeared in substantial quantities in the extracellular fluid, in the presence of all three proteins, while extracellular glycylprolyl dipeptidase was only produced in the presence of collagen. The data supports the hypothesis that P. gingivalis has the capacity to regulate the synthesis and secretion of hydrolytic enzymes in response to environmental conditions. This may be of considerable importance in the development of periodontal disease caused by P. gingivalis.Keywords
This publication has 19 references indexed in Scilit:
- Proposal for Reclassification of Bacteroides asaccharolyticus, Bacteroides gingivalis, and Bacteroides endodontalis in a New Genus, PorphyromonasInternational Journal of Systematic and Evolutionary Microbiology, 1988
- Enhanced Growth of Complex Communities of Dental Plaque Bacteria in Mucin-Limited Continuous CultureMicrobial Ecology in Health & Disease, 1988
- Increased Degradative Enzyme Production by Dental Plaque Bacteria in Mucin-limited Continuous CultureMicrobial Ecology in Health & Disease, 1988
- Utilization of glucose and amino acids byBacteroides intermedius andBacteroides gingivalisCurrent Microbiology, 1987
- Glycylprolyl Dipeptidylaminopeptidase from Bacteroides gingivalisJournal of Dental Research, 1985
- OCCURRENCE AND NATURE OF BACTERIAL IgA PROTEASES*Annals of the New York Academy of Sciences, 1983
- Genetic Heterogeneity in Bacteroides asaccharolyticus (Holdeman and Moore 1970) Finegold and Barnes 1977 (Approved Lists, 1980) and Proposal of Bacteroides gingivalis sp. nov. and Bacteroides macacae (Slots and Genco) comb. nov.International Journal of Systematic and Evolutionary Microbiology, 1980
- Growth of Cutaneous Propionibacteria on Synthetic Medium; Growth Yields and Exoenzyme ProductionJournal of Applied Bacteriology, 1979
- Report of the ICSB Taxonomic Subcommittee on Gram-Negative Anaerobic Rods: Proposal That the Saccharolytic and Asaccharolytic Strains at Present Classified in the Species Bacteroides melaninogenicus (Oliver and Wherry) Be Reclassified in Two Species as Bacteroides melaninogenicus and Bacteroides asaccharolyticusInternational Journal of Systematic and Evolutionary Microbiology, 1977
- Comparison of the Biochemical Properties of Bacteroides melaninogenicus from Human Dental Plaque and Other SitesJournal of Applied Bacteriology, 1976