Retrospective and Prospective Verification of the Cepheid Xpert Influenza Virus Assay

Abstract
We performed a retrospective ( n = 121) and prospective ( n = 305) verification of the Cepheid Xpert Flu assay to determine its performance characteristics. The overall sensitivity and specificity were 93% and 100%, respectively. Nasopharyngeal specimen sensitivities were 100% for seasonal influenza A/H1 virus and influenza A/H3 virus, 90% for influenza A/2009/H1N1 virus, and 95% for influenza B virus.