Dynamics of the cytosolic chelatable iron pool of K562 cells
Open Access
- 18 March 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 382 (3), 304-308
- https://doi.org/10.1016/0014-5793(96)00190-1
Abstract
The labile iron pool of cells (LIP) constitutes the primary source of metabolic and catalytically reactive iron in the cytosol. We studied LIP homeostasis in K562 cells using the fluorescent metal‐sensitive probe calcein. Following brief exposure to iron(II) salts or to oxidative or reductive stress, LIP rose by up to 120% relative to the normal level of 350 nM. However, the rate of recovery to normal LIP level differed markedly with each treatment (respective of 27, 65–88 and ≤17 min). We show that the capacity of K562 cells to adjust LIP levels is highly dependent on the origin of the LIP increase and on the pre‐existing cellular iron status.Keywords
This publication has 26 references indexed in Scilit:
- Iron Acquired from Transferrin by K562 Cells Is Delivered into a Cytoplasmic Pool of Chelatable Iron(II)Published by Elsevier BV ,1995
- Modulation by iron loading and chelation of the uptake of non-transferrin-bound iron by human liver cellsBiochimica et Biophysica Acta (BBA) - General Subjects, 1995
- Ascorbic Acid Enhances Iron-induced Ferritin Translation in Human Leukemia and Hepatoma CellsJournal of Biological Chemistry, 1995
- The increased susceptibility to hydrogen peroxide of the (post-)ischemic rat heart is associated with the magnitude of the low molecular weight iron poolFree Radical Biology & Medicine, 1994
- Hypothermia triggers iron-dependent lipoperoxidative damage in the isolated rat heartFree Radical Biology & Medicine, 1994
- Evaluation of the iron chelation potential of hydrazones of pyridoxal, salicylaldehyde and 2-hydroxy-1-naphthylaldehyde using the hepatocyte in cultureHepatology, 1992
- Effect of diquat on the distribution of iron in rat liverToxicology and Applied Pharmacology, 1988
- Iron-Chelating TherapyCRC Critical Reviews in Clinical Laboratory Sciences, 1988
- Reductive mobilisation of ferritin ironEuropean Journal of Biochemistry, 1985
- The effect of various chelating agents on the mobilization of iron from reticulocytes in the presence and absence of pyridoxal isonicotinoyl hydrazoneBiochimica et Biophysica Acta (BBA) - General Subjects, 1984