Dispersed donor salivary gland cells are widely distributed in the recipient gland when infused up the ductal tree
- 8 January 2010
- journal article
- Published by Taylor & Francis Ltd in Biotechnic & Histochemistry
- Vol. 84 (6), 253-260
- https://doi.org/10.3109/10520290903081377
Abstract
The salivary glands often are severely and permanently damaged by therapeutic irradiation for cancer of the head and neck. The markedly reduced quantity and quality of saliva results in greatly increased susceptibility to dental caries and infection of the oral mucosa and alveolar bone. Recently, subcapsular injection of cultured mouse salivary gland cells has achieved a significant degree of regeneration in a previously irradiated mouse salivary gland; however, the recovery was limited to one lobule. We describe here a method for delivering donor rat salivary gland cells via the main duct that distributes several thousand cells throughout the recipient rat's salivary gland. The donated cells exhibited the cytodifferentiation of the structures in which they lodged, i.e., acini, granular convoluted tubules, and the several types of ducts. This method may facilitate the simultaneous functional recovery of almost all of the lobules of irradiated rat salivary glands.Keywords
This publication has 21 references indexed in Scilit:
- Rescue of Salivary Gland Function after Stem Cell Transplantation in Irradiated GlandsPLOS ONE, 2008
- On approaches to the functional restoration of salivary glands damaged by radiation therapy for head and neck cancer, with a review of related aspects of salivary gland morphology and developmentBiotechnic & Histochemistry, 2008
- Mobilization of Bone Marrow Stem Cells by Granulocyte Colony-Stimulating Factor Ameliorates Radiation-Induced Damage to Salivary GlandsClinical Cancer Research, 2006
- Cationic liposome-mediated gene transfer to rat salivary epithelial cellsin vitro andin vivoThe Journal of Gene Medicine, 2000
- A new rat repetitive DNA family shows preferential localization on chromosome 3, 12 and Y after fluorescence in situ hybridization and contains a subfamily which is Y chromosome specificCytogenetic and Genome Research, 1995
- Accumulation and localization of two adult acinar cell secretory proteins during development of the rat submandibular glandJournal of Anatomy, 1991
- Light and electron microscopic immunolocalization of rat submandibular gland mucin glycoprotein and glutamine/glutamic acid-rich proteins.Journal of Histochemistry & Cytochemistry, 1989
- Cell death and cell proliferation during atrophy of the rat parotid gland induced by duct obstructionThe Journal of Pathology, 1987
- Submaxillary gland recovery from obstruction. I. Overall changes and electron microscopic alterations of granular duct cellsJournal of Ultrastructure Research, 1971
- THE SPECIAL VALUE OF METHODS THAT COLOR BOTH ACIDIC AND VICINAL HYDROXYL GROUPS IN THE HISTOCHEMICAL STUDY OF MUCINS. WITH REVISED DIRECTIONS FOR THE COLLOIDAL IRON STAIN, THE USE OF ALCIAN BLUE G8X AND THEIR COMBINATIONS WITH THE PERIODIC ACID‐SCHIFF REACTION*Annals of the New York Academy of Sciences, 1963