Arrested Protein Synthesis Increases Persister-Like Cell Formation
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Open Access
- 1 March 2013
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 57 (3), 1468-1473
- https://doi.org/10.1128/aac.02135-12
Abstract
Biofilms are associated with a wide variety of bacterial infections and pose a serious problem in clinical medicine due to their inherent resilience to antibiotic treatment. Within biofilms, persister cells comprise a small bacterial subpopulation that exhibits multidrug tolerance to antibiotics without undergoing genetic change. The low frequency of persister cell formation makes it difficult to isolate and study persisters, and bacterial persistence is often attributed to a quiescent metabolic state induced by toxins that are regulated through toxin-antitoxin systems. Here we mimic toxins via chemical pretreatments to induce high levels of persistence (10 to 100%) from an initial population of 0.01%. Pretreatment of Escherichia coli with (i) rifampin, which halts transcription, (ii) tetracycline, which halts translation, and (iii) carbonyl cyanide m-chlorophenylhydrazone, which halts ATP synthesis, all increased persistence dramatically. Using these compounds, we demonstrate that bacterial persistence results from halted protein synthesis and from environmental cues.Keywords
This publication has 48 references indexed in Scilit:
- A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoSNature Chemical Biology, 2012
- Signaling-mediated bacterial persister formationNature Chemical Biology, 2012
- Heterogeneous bacterial persisters and engineering approaches to eliminate themCurrent Opinion in Microbiology, 2011
- Bacterial persistence by RNA endonucleasesProceedings of the National Academy of Sciences of the United States of America, 2011
- Antitoxin MqsA helps mediate the bacterial general stress responseNature Chemical Biology, 2011
- Structure of the Escherichia coli Antitoxin MqsA (YgiT/b3021) Bound to Its Gene Promoter Reveals Extensive Domain Rearrangements and the Specificity of Transcriptional RegulationJournal of Biological Chemistry, 2011
- YeeV is an Escherichia coli toxin that inhibits cell division by targeting the cytoskeleton proteins, FtsZ and MreBMolecular Microbiology, 2010
- Toxins Hha and CspD and small RNA regulator Hfq are involved in persister cell formation through MqsR in Escherichia coliBiochemical and Biophysical Research Communications, 2009
- MqsR, a Crucial Regulator for Quorum Sensing and Biofilm Formation, Is a GCU-specific mRNA Interferase in Escherichia coliPublished by Elsevier BV ,2009
- Construction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collectionMolecular Systems Biology, 2006