Maintaining dimensions and mechanical properties of ionically crosslinked alginate hydrogel scaffolds in vitro
- 23 July 2007
- journal article
- Published by Wiley in Journal of Biomedical Materials Research Part A
- Vol. 84A (4), 899-907
- https://doi.org/10.1002/jbm.a.31375
Abstract
Ionically crosslinked alginate hydrogels are attractive scaffolds because of their biocompatibility and mild gelation reaction that allows for gentle cell incorporation. However, the instability of ionically crosslinked hydrogels in an aqueous environment is a challenge that limits their application. This report presents a novel method to control the dimensions and mechanical properties of ionically crosslinked hydrogels via control of the ionic concentration of the medium. Homogeneous calcium‐alginate gels were incubated in physiological saline baths adjusted to specific calcium ion concentrations. Swelling and shrinking occurred at low and high ionic concentrations of the medium, respectively, while an “optimal” intermediate calcium ion concentration of the medium was found to maintain original size and shape of the hydrogel. This optimal calcium ion concentration was found to be a function of crosslinking density and polymer concentration of the hydrogel and chemical composition of the alginate. The effects of optimal and high calcium ion concentrations of the medium on swelling behavior, calcium content, dry weight, and mechanical properties of the immersed hydrogels were investigated. It was found that the resulting hydrogel composition and mechanical properties depended on not only the calcium concentration of the medium, but also the crosslinking density and polymer concentration of the gel. In an 8‐week experiment, controlled dimensions and mechanical properties of alginate gels in an aqueous environment were demonstrated. This new technique significantly enhances the potential of alginate hydrogels for tissue engineering and other biomedical applications. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008Keywords
This publication has 36 references indexed in Scilit:
- Biodegradable and photocrosslinkable polyphosphoester hydrogelBiomaterials, 2005
- Injectable cartilage tissue engineeringExpert Opinion on Biological Therapy, 2004
- Controlled Degradation and Mechanical Behavior of Photopolymerized Hyaluronic Acid NetworksBiomacromolecules, 2004
- Scaffolds for tissue fabricationMaterials Today, 2004
- Photocrosslinkable polysaccharides based on chondroitin sulfateJournal of Biomedical Materials Research Part A, 2003
- Photopolymerizable hydrogels for tissue engineering applicationsBiomaterials, 2002
- Synthesis and characterization of photocrosslinkable, degradable poly(vinyl alcohol)-based tissue engineering scaffolds.Biomaterials, 2002
- Hydrogels for biomedical applicationsAdvanced Drug Delivery Reviews, 2001
- Ionically crosslinked alginate hydrogels as scaffolds for tissue engineering: Part 1. Structure, gelation rate and mechanical propertiesBiomaterials, 2001
- Hydrogels in pharmaceutical formulationsEuropean Journal of Pharmaceutics and Biopharmaceutics, 2000