Porcine Arterivirus Attachment to the Macrophage-Specific Receptor Sialoadhesin Is Dependent on the Sialic Acid-Binding Activity of the N-Terminal Immunoglobulin Domain of Sialoadhesin
- 1 September 2007
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 81 (17), 9546-9550
- https://doi.org/10.1128/jvi.00569-07
Abstract
The sialic acid-binding lectin sialoadhesin (Sn) is a macrophage-restricted receptor for porcine reproductive and respiratory syndrome virus (PRRSV). To investigate the importance of pSn sialic acid-binding activity for PRRSV infection, an R 116 -to-E mutation was introduced in the predicted sialic acid-binding domain of pSn, resulting in a mutant, pSn RE , that could not bind sialic acids. PSn, but not pSn RE , allowed PRRSV binding and internalization. These data show that the sialic acid-binding activity of pSn is essential for PRRSV attachment to pSn and thus identifies the variable, N-terminal domain of Sn as a PRRSV binding domain.Keywords
This publication has 28 references indexed in Scilit:
- The antigen recognized by MOMA-I is sialoadhesinImmunology Letters, 2006
- Nidovirales: Evolving the largest RNA virus genomeVirus Research, 2006
- Identification of porcine alveolar macrophage glycoproteins involved in infection of porcine respiratory and reproductive syndrome virusArchiv für die gesamte Virusforschung, 2003
- A summary of taxonomic changes recently approved by ICTVArchiv für die gesamte Virusforschung, 2002
- Effects of origin and state of differentiation and activation of monocytes/macrophages on their susceptibility to porcine reproductive and respiratory syndrome virus (PRRSV)Archiv für die gesamte Virusforschung, 1997
- Virus quantification and identification of cellular targets in the lungs and lymphoid tissues of pigs at different time intervals after inoculation with porcine reproductive and respiratory syndrome virus (PRRSV)Veterinary Microbiology, 1997
- Expression, crystallization, and preliminary X-ray analysis of a sialic acid-binding fragment of sialoadhesin in the presence and absence of ligandProtein Science, 1997
- Characterization of the Sialic Acid-binding Site in Sialoadhesin by Site-directed MutagenesisJournal of Biological Chemistry, 1996
- The Amino-terminal Immunoglobulin-like Domain of Sialoadhesin Contains the Sialic Acid Binding SitePublished by Elsevier BV ,1995
- Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell lineArchiv für die gesamte Virusforschung, 1993