Genome and Proteome of Campylobacter jejuni Bacteriophage NCTC 12673
- 1 December 2011
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 77 (23), 8265-8271
- https://doi.org/10.1128/aem.05562-11
Abstract
Campylobacter jejuni continues to be the leading cause of bacterial food-borne illness worldwide, so improvements to current methods used for bacterial detection and disease prevention are needed. We describe here the genome and proteome of C. jejuni bacteriophage NCTC 12673 and the exploitation of its receptor-binding protein for specific bacterial detection. Remarkably, the 135-kb Myoviridae genome of NCTC 12673 differs greatly from any other proteobacterial phage genome described (including C. jejuni phages CP220 and CPt10) and instead shows closest homology to the cyanobacterial T4-related myophages. The phage genome contains 172 putative open reading frames, including 12 homing endonucleases, no visible means of packaging, and a putative trans -splicing intein. The phage DNA appears to be strongly associated with a protein that interfered with PCR amplification and estimation of the phage genome mass by pulsed-field gel electrophoresis. Identification and analyses of the receptor-binding protein (Gp48) revealed features common to the Salmonella enterica P22 phage tailspike protein, including the ability to specifically recognize a host organism. Bacteriophage receptor-binding proteins may offer promising alternatives for use in pathogen detection platforms.Keywords
This publication has 34 references indexed in Scilit:
- Orally Administered P22 Phage Tailspike Protein Reduces Salmonella Colonization in Chickens: Prospects of a Novel Therapy against Bacterial InfectionsPLOS ONE, 2010
- Bacteriophage tailspike proteins as molecular probes for sensitive and selective bacterial detectionBiosensors and Bioelectronics, 2010
- The in vivo efficacy of two administration routes of a phage cocktail to reduce numbers of Campylobacter coli and Campylobacter jejuni in chickensBMC Microbiology, 2010
- Protein kinetic stabilityBiophysical Chemistry, 2010
- Evidence for a lineage of virulent bacteriophages that target CampylobacterBMC Genomics, 2010
- Classification of Myoviridae bacteriophages using protein sequence similarityBMC Microbiology, 2009
- Outer membrane vesicle-mediated release of cytolethal distending toxin (CDT) from Campylobacter jejuniBMC Microbiology, 2009
- Protein structure prediction on the Web: a case study using the Phyre serverNature Protocols, 2009
- Short- and Long-term Effects of Bacterial Gastrointestinal InfectionsEmerging Infectious Diseases, 2008
- Structural Basis of Protein Kinetic Stability: Resistance to Sodium Dodecyl Sulfate Suggests a Central Role for Rigidity and a Bias Toward β-Sheet StructureBiochemistry, 2004