Interdependence of Cell Growth and Gene Expression: Origins and Consequences
Top Cited Papers
- 19 November 2010
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 330 (6007), 1099-1102
- https://doi.org/10.1126/science.1192588
Abstract
In bacteria, the rate of cell proliferation and the level of gene expression are intimately intertwined. Elucidating these relations is important both for understanding the physiological functions of endogenous genetic circuits and for designing robust synthetic systems. We describe a phenomenological study that reveals intrinsic constraints governing the allocation of resources toward protein synthesis and other aspects of cell growth. A theory incorporating these constraints can accurately predict how cell proliferation and gene expression affect one another, quantitatively accounting for the effect of translation-inhibiting antibiotics on gene expression and the effect of gratuitous protein expression on cell growth. The use of such empirical relations, analogous to phenomenological laws, may facilitate our understanding and manipulation of complex biological systems before underlying regulatory circuits are elucidated.Keywords
This publication has 25 references indexed in Scilit:
- Growth Rate-Dependent Global Effects on Gene Expression in BacteriaCell, 2009
- Growth landscape formed by perception and import of glucose in yeastNature, 2009
- Invariant Distribution of Promoter Activities in Escherichia coliPLoS Computational Biology, 2009
- Emergent bistability by a growth-modulating positive feedback circuitNature Chemical Biology, 2009
- Predicting Cellular Growth from Gene Expression SignaturesPLoS Computational Biology, 2009
- The Cost of Expression of Escherichia coli lac Operon Proteins Is in the Process, Not in the ProductsGenetics, 2008
- Metabolic load and heterologous gene expressionBiotechnology Advances, 1995
- Synthesis of Proteins in Escherichia coli is Limited by the Concentration of Free Ribosomes: Expression from Reporter Genes does not always Reflect Functional mRNA LevelsJournal of Molecular Biology, 1993
- Growth rate of polypeptide chains as a function of the cell growth rate in a mutant of Escherichia coli 15Journal of Molecular Biology, 1971
- THE GROWTH OF BACTERIAL CULTURESAnnual Review of Microbiology, 1949