Shape-Specific, Monodisperse Nano-Molding of Protein Particles
- 1 April 2008
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 130 (16), 5438-5439
- https://doi.org/10.1021/ja8014428
Abstract
Herein we report nano-molding proteins for the fabrication of protein PRINT particles of monodisperse size and shape. Lyophilized protein particles are generally highly dispersed in particle size, aggregated, and often made through costly and complicated processes. Attempts to engineer monodisperse, discrete protein particles using wet-milling, spray−freeze−drying, microemulsion, or super critical fluid methods have realized little success. The PRINT technology enables a gentle, facile route to monodisperse particles of 100% protein as small as 200 nm cylinders. Protein PRINT particles of any shape and size are effortlessly achievable. Our research efforts include making PRINT particles composed of albumin and albumin 0.5 wt % siRNA, and Abraxane, the gold standard therapeutic used in metastatic breast cancer.Keywords
This publication has 15 references indexed in Scilit:
- Study of the Interactions between Protein-Imprinted Hydrogels and Their TemplatesLangmuir, 2007
- Straightforward Protein Immobilization on Sylgard 184 PDMS Microarray SurfaceLangmuir, 2007
- Powder formation by atmospheric spray-freeze-dryingPowder Technology, 2006
- Nanoporous Protein Particles Through Templating Mesoporous Silica SpheresAdvanced Materials, 2006
- Fabrication of nanopatterned films of bovine serum albumin and staphylococcal protein A using latex particle lithographyThe Analyst, 2005
- Fabrication of Protein/Silica Core–Shell Nanoparticles by Microemulsion‐Based Molecular WrappingAdvanced Materials, 2004
- The production of protein-loaded microparticles by supercritical fluid enhanced mixing and sprayingJournal of Controlled Release, 2004
- Optimization of the preparation process for human serum albumin (HSA) nanoparticlesInternational Journal of Pharmaceutics, 2003
- Protein spray freeze drying. 2. Effect of formulation variables on particle size and stabilityJournal of Pharmaceutical Sciences, 2002
- Supercritical fluid processing of proteins: I: Lysozyme precipitation from organic solutionEuropean Journal of Pharmaceutical Sciences, 2000