3D Motion of DNA-Au Nanoconjugates in Graphene Liquid Cell Electron Microscopy
- 20 August 2013
- journal article
- research article
- Published by American Chemical Society (ACS) in Nano Letters
- Vol. 13 (9), 4556-4561
- https://doi.org/10.1021/nl402694n
Abstract
Liquid-phase transmission electron microscopy (TEM) can probe and visualize dynamic events with structural or functional details at the nanoscale in a liquid medium. Earlier efforts have focused on the growth and transformation kinetics of hard material systems, relying on their stability under electron beam. Our recently developed graphene liquid cell technique pushed the spatial resolution of such imaging to the atomic scale but still focused on growth trajectories of metallic nanocrystals. Here, we adopt this technique to imaging three-dimensional (3D) dynamics of soft materials instead, double strand (dsDNA) connecting Au nanocrystals as one example, at nanometer resolution. We demonstrate first that a graphene liquid cell can seal an aqueous sample solution of a lower vapor pressure than previously investigated well against the high vacuum in TEM. Then, from quantitative analysis of real time nanocrystal trajectories, we show that the status and configuration of dsDNA dictate the motions of linked nanocrystals throughout the imaging time of minutes. This sustained connecting ability of dsDNA enables this unprecedented continuous imaging of its dynamics via TEM. Furthermore, the inert graphene surface minimizes sample–substrate interaction and allows the whole nanostructure to rotate freely in the liquid environment; we thus develop and implement the reconstruction of 3D configuration and motions of the nanostructure from the series of 2D projected TEM images captured while it rotates. In addition to further proving the nanoconjugate structural stability, this reconstruction demonstrates 3D dynamic imaging by TEM beyond its conventional use in seeing a flattened and dry sample. Altogether, we foresee the new and exciting use of graphene liquid cell TEM in imaging 3D biomolecular transformations or interaction dynamics at nanometer resolution.This publication has 32 references indexed in Scilit:
- Imaging Protein Structure in Water at 2.7 nm Resolution by Transmission Electron MicroscopyBiophysical Journal, 2012
- Charged Nanoparticle Dynamics in Water Induced by Scanning Transmission Electron MicroscopyLangmuir, 2012
- Controlled Growth of Nanoparticles from Solution with In Situ Liquid Transmission Electron MicroscopyNano Letters, 2011
- Fully Hydrated Yeast Cells Imaged with Electron MicroscopyBiophysical Journal, 2011
- Fast, three-dimensional super-resolution imaging of live cellsNature Methods, 2011
- Visualizing Gold Nanoparticle Uptake in Live Cells with Liquid Scanning Transmission Electron MicroscopyNano Letters, 2011
- Correlative Fluorescence Microscopy and Scanning Transmission Electron Microscopy of Quantum-Dot-Labeled Proteins in Whole Cells in LiquidACS Nano, 2010
- Probing the Conformational Distributions of Subpersistence Length DNABiophysical Journal, 2009
- Pyramidal and Chiral Groupings of Gold Nanocrystals Assembled Using DNA ScaffoldsJournal of the American Chemical Society, 2009
- Gold nanoparticle–protein arrays improve resolution for cryo-electron microscopyJournal of Structural Biology, 2008