Synthesis and Analytical Use of 3-Carboxypropionyl-alanyl-alanyl-valine-4-nitroanilide: A Specific Substrate for Human Leukocyte Elastase
- 1 January 1980
- journal article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 361 (2), 1413-1416
- https://doi.org/10.1515/bchm2.1980.361.2.1413
Abstract
A simple synthesis is described for 3-carboxypropionyl-Ala-Ala-Val-4-nitroanilide, a convenient and very specific substrate for human leukocyte elastase (Km = 1.0mM, kcat = 8.7 s-1). The substrate does not undergo appreciable spontaneous hydrolysis. It is not cleaved by trypsin or chymotrypsin and only rather slowly by porcine pancreatic elastase (Km = 9.1mM, kcat = 1.4 s-1).Keywords
This publication has 1 reference indexed in Scilit:
- On the size of the active site in proteases. I. PapainBiochemical and Biophysical Research Communications, 1967