Performance Characteristics of a Multiplex Flow Immunoassay for Detection of IgG-Class Antibodies to Measles, Mumps, Rubella, and Varicella-Zoster Viruses in Presumptively Immune Health Care Workers
- 1 April 2020
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 58 (4)
- https://doi.org/10.1128/JCM.00136-20
Abstract
Immunity to measles, mumps, rubella, and varicella-zoster viruses (VZV; MMRV) is a common condition of employment for health care workers (HCWs) to ensure compliance with national standards and state laws. When documentation of complete vaccination or laboratory-confirmed infection is not available, Advisory Committee on Immunization Practices (ACIP) criteria are used to guide vaccination or anti-MMRV IgG testing. We assessed the performance of the BioPlex 2200 MMRV IgG multiplex flow immunoassay (MFI; Bio-Rad Laboratories, Hercules, CA) and matched immunofluorescence assays (IFAs; MBL Bion, Des Plaines, IL) in 220 HCWs categorized by ACIP criteria for presumptive immunity to MMRV. Among HCWs presumptively immune to measles, mumps, rubella, and VZV, the Bio-Rad MFI was positive in 77.3, 85.4, 84.3, and 91.1% of HCWs, respectively. Comparatively, the Bion IFA was positive in 92.9, 91.1, and 93.5% of HCWs presumptively immune to measles, mumps, and VZV (a rubella IFA was unavailable). Among HCWs fully vaccinated against measles, mumps, and VZV, Bio-Rad MFI/Bion IFA positivity rates were 77.4%/93%, 84.8%/ 90.7%, and 54.5%/90.9%, respectively. The Bio-Rad MFI was positive in 83.7% of HCWs fully vaccinated against rubella. For HCWs whose last vaccination event occurred within 15 years of enrollment, 83.3, 93.3, and 74.2% were positive by the BioRad measles, mumps, and rubella IgG MFIs, respectively. We show significantly decreased Bio-Rad MFI sensitivity for detection of anti-measles and anti-mumps IgG-class antibodies in presumptively immune or fully vaccinated HCWs. Although negative results typically prompt revaccination, failure to recognize presumptive immunity in individuals unable to receive live, attenuated vaccines may have employment implications.Keywords
Funding Information
- Mayo Clinic
This publication has 24 references indexed in Scilit:
- Development of a varicella-zoster virus neutralization assay using a glycoprotein K antibody enzyme-linked immunosorbent spot assayJournal of Virological Methods, 2014
- Evaluation of the Bio-Rad BioPlex Measles, Mumps, Rubella, and Varicella-Zoster Virus IgG Multiplex Bead ImmunoassayClinical and Vaccine Immunology, 2011
- Booster Vaccinations: Can Immunologic Memory Outpace Disease Pathogenesis?PEDIATRICS, 2009
- Persistence of Rubella Antibodies after 2 Doses of Measles‐Mumps‐Rubella VaccineThe Journal of Infectious Diseases, 2009
- Persistence of Mumps Antibodies after 2 Doses of Measles‐Mumps‐Rubella VaccineThe Journal of Infectious Diseases, 2009
- Persistence of Measles, Mumps, and Rubella Antibodies in an MMR‐Vaccinated Cohort: A 20‐Year Follow‐upThe Journal of Infectious Diseases, 2008
- Use and Limitations of Varicella‐Zoster Virus–Specific Serological Testing to Evaluate Breakthrough Disease in Vaccinees and to Screen for Susceptibility to VaricellaThe Journal of Infectious Diseases, 2008
- Persistence of Measles Antibodies After 2 Doses of Measles Vaccine in a Postelimination EnvironmentArchives of Pediatrics & Adolescent Medicine, 2007
- Mumps Virus-Specific Antibody Titers from Pre-Vaccine Era Sera: Comparison of the Plaque Reduction Neutralization Assay and Enzyme ImmunoassaysJournal of Clinical Microbiology, 2005
- Measles Antibody: Reevaluation of Protective TitersThe Journal of Infectious Diseases, 1990