Janus Emulsions for the Detection of Bacteria
Open Access
- 23 March 2017
- journal article
- research article
- Published by American Chemical Society (ACS) in ACS Central Science
- Vol. 3 (4), 309-313
- https://doi.org/10.1021/acscentsci.7b00021
Abstract
Janus emulsion assays that rely on carbohydrate–lectin binding for the detection of Escherichia coli bacteria are described. Surfactants containing mannose are self-assembled at the surface of Janus droplets to produce particles with lectin binding sites. Janus droplets orient in a vertical direction as a result of the difference in densities between the hydrocarbon and fluorocarbon solvents. Binding of lectin to mannose(s) causes agglutination and a tilted geometry. The distinct optical difference between naturally aligned and agglutinated Janus droplets produces signals that can be detected quantitatively. The Janus emulsion assay sensitively and selectively binds to E. coli at 104 cfu/mL and can be easily prepared with long-time stability. It provides the basis for the development of inexpensive portable devices for fast, on-site pathogen detection.Funding Information
- Army Research Office (W911NF-13-D-0001)
- Deutsche Forschungsgemeinschaft (SFB-TR 84)
- Max-Planck-Gesellschaft
- Alexander von Humboldt-Stiftung
- Abdul Latif Jameel World Water and Food Security Lab, Massachusetts Institute of Technology
This publication has 20 references indexed in Scilit:
- Surface organelles assembled by secretion systems of Gram-negative bacteria: diversity in structure and functionFEMS Microbiology Reviews, 2012
- Optimization of fixation methods for observation of bacterial cell morphology and surface ultrastructures by atomic force microscopyApplied Microbiology and Biotechnology, 2011
- Hexameric Supramolecular Scaffold Orients Carbohydrates To Sense BacteriaJournal of the American Chemical Society, 2011
- Methods for Rapid Detection of Foodborne Pathogens: An OverviewAmerican Journal of Food Technology, 2011
- Structurally Controlled Bio‐hybrid Materials Based on Unidirectional Association of Anisotropic Microparticles with Human Endothelial CellsAdvanced Materials, 2009
- A Streptavidin−Protein Cage Janus Particle for Polarized Targeting and Modular FunctionalizationJournal of the American Chemical Society, 2009
- Synthesis of Fluorescent Carbohydrate-Protected Au Nanodots for Detection of Concanavalin A and Escherichia coliAnalytical Chemistry, 2009
- Structurally Distinct Requirements for Binding of P-selectin Glycoprotein Ligand-1 and Sialyl Lewis x to Anaplasma phagocytophilum and P-selectinJournal of Biological Chemistry, 2003
- Preparation of alkyl α- and β-d-glucopyranosides, thermotropic properties and X-ray analysisCarbohydrate Research, 1998
- Immunological methods for detection of foodborne pathogens and their toxinsInternational Journal of Food Microbiology, 1991