Noncanonical frizzled signaling regulates cell polarity of growth plate chondrocytes
Open Access
- 1 April 2009
- journal article
- Published by The Company of Biologists in Development
- Vol. 136 (7), 1083-1092
- https://doi.org/10.1242/dev.023820
Abstract
Bone growth is driven by cell proliferation and the subsequent hypertrophy of chondrocytes arranged in columns of discoid cells that resemble stacks of coins. However, the molecular mechanisms that direct column formation and the importance of columnar organization to bone morphogenesis are not known. Here,we show in chick that discoid proliferative chondrocytes orient the division plane to generate daughter cells that are initially displaced laterally and then intercalate into the column. Downregulation of frizzled (Fzd) signaling alters the dimensions of long bones and produces cell-autonomous changes in proliferative chondrocyte organization characterized by arbitrary division planes and altered cell stacking. These defects are phenocopied by disruption of noncanonical effector pathways but not by inhibitors of canonical Fzd signaling. These findings demonstrate that the regulation of cell polarity and cell arrangement by noncanonical Fzd signaling plays important roles in generating the unique morphological characteristics that shape individual cartilage elements.This publication has 78 references indexed in Scilit:
- Involvement of RhoA, ROCK I and myosin II in inverted orientation of epithelial polarityEMBO Reports, 2008
- Zebrafish colgate/hdac1 functions in the non-canonical Wnt pathway during axial extension and in Wnt-independent branchiomotor neuron migrationMechanisms of Development, 2007
- Planar cell polarity: one or two pathways?Nature Reviews Genetics, 2007
- Wnt5a functions in planar cell polarity regulation in miceDevelopmental Biology, 2007
- Asymmetric localizations of LIN-17/Fz and MIG-5/Dsh are involved in the asymmetric B cell division in C. elegansDevelopmental Biology, 2007
- Integrin-mediated adhesion orients the spindle parallel to the substratum in an EB1- and myosin X-dependent mannerThe EMBO Journal, 2007
- Runx2 inhibits chondrocyte proliferation and hypertrophy through its expression in the perichondriumGenes & Development, 2006
- Defective planar cell polarity in polycystic kidney diseaseNature Genetics, 2005
- Developmental regulation of the growth plateNature, 2003
- A series of normal stages in the development of the chick embryoDevelopmental Dynamics, 1992