Generalized single-particle cryo-EM – a historical perspective
Open Access
- 12 November 2015
- journal article
- review article
- Published by Oxford University Press (OUP) in Microscopy
- Vol. 65 (1), 3-8
- https://doi.org/10.1093/jmicro/dfv358
Abstract
This is a brief account of the earlier history of single-particle cryo-EM of biological molecules lacking internal symmetry, which goes back to the mid-seventies. The emphasis of this review is on the mathematical concepts and computational approaches. It is written as the field experiences a turning point in the wake of the introduction of digital cameras capable of single electron counting, and near-atomic resolution can be reached even for smaller molecules.Keywords
Funding Information
- HHMI
- NIH (R01 GM 29169)
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