Genetics of drought tolerance during seed germination in tomato: inheritance and QTL mapping
- 1 August 2003
- journal article
- Published by Canadian Science Publishing in Genome
- Vol. 46 (4), 536-545
- https://doi.org/10.1139/g03-035
Abstract
A BC1population (N = 1000) of an F1hybrid between a stress-sensitive Lycopersicon esculentum breeding line (NC84173; maternal and recurrent parent) and a germination stress-tolerant Lycopersicon pimpinellifolium accession (LA722) was evaluated for seed germination rate under drought stress (DS) (14% w/v polyethyleneglycol-8000, water potential approximately –680 kPa), and the most rapidly germinating seeds (first 3% to germinate) were selected. The 30 selected BC1seedlings were grown to maturity and self pollinated to produce BC1S1progeny seeds. Twenty of the 30 selected BC1S1progeny families were evaluated for germination rate under DS and their average performance was compared with that of a "nonselected" BC1S1population of the same cross. Results indicated that selection for rapid germination under DS significantly improved progeny germination rate under DS (selection gain = 19.6%), suggesting a realized heritability of 0.47 for rate of germination under DS in this population. The 30 selected BC1plants were subjected to restriction fragment length polymorphism (RFLP) analysis, and marker allele frequencies for 119 RFLP markers which spanned 1153 cM of the 12 tomato chromosomes were determined. A distributional extreme marker analysis, which measures statistical differences in marker allele frequencies between a selected and a nonselected population, detected four quantitative trait loci (QTLs) for rate of germination under DS in this population. Of these, two QTLs, located on chromosomes 1 and 9, were contributed by the L. pimpinellifolium donor parent and had larger effects than the other two QTLs, located on chromosomes 8 and 12, which were contributed by the L. esculentum recurrent parent. A few BC1S1families were identified with all or most of the identified QTLs and with germination rates comparable with that of LA722. These families should be useful for the development of germination drought-tolerant tomato lines using marker-assisted selection (MAS). The overall results indicate that drought tolerance during seed germination in tomato is genetically controlled and potentially could be improved by directional phenotypic selection or MAS.Key words: abiotic stress, distributional extreme analysis, heritability, selective genotyping, stress tolerance.Keywords
This publication has 42 references indexed in Scilit:
- A molecular linkage map of tomato based on a cross between Lycopersicon esculentum andL. pimpinellifolium and its comparison with other molecular maps of tomatoGenome, 1999
- Genetics of Salt and Cold Tolerance in Tomato: Quantitative Analysis and QTL Mapping.Plant Biotechnology, 1999
- Mapping of QTLs for lycopene and other fruit traits in a Lycopersicon esculentum × L. pimpinellifolium cross and comparison of QTLs across tomato speciesMolecular Breeding, 1999
- Drought as a challenge for the plant breederPlant Growth Regulation, 1996
- Response to Selection for Salt Tolerance during Germination in Tomato Seed Derived from PI 174263Journal of the American Society for Horticultural Science, 1996
- Assessment of RAPD marker frequencies over cycles of recurrent selection for pigment concentration and percent solids in red beet (Beta vulgaris L.)Molecular Breeding, 1996
- Molecular Markers in Plant Improvement: Manipulation of Genes Affecting Quantitative TraitsCrop Science, 1993
- Selective genotyping for determination of linkage between a marker locus and a quantitative trait locusTheoretical and Applied Genetics, 1992
- Majority of random cDNA clones correspond to single loci in the tomato genomeMolecular Genetics and Genomics, 1986
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1983