Measurement of rat brown-adipose-tissue mitochondrial uncoupling protein by radioimmunoassay: Increased concentration after cold acclimation

Abstract
A specific antiserum was raised against the 32,000-MW uncoupling protein from the mitochondria of rat brown adipose tissue and a sensitive radioimmunoassay for the protein was developed. The uncoupling protein is present in large amounts in brown adipose tissue; its concentration is increased substantially by cold acclimation. The protein was not detected in the liver, heart, or parametrial white adipose tissue of rats.

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