Acidic Shell Proteins of the Mediterranean Fan Mussel Pinna nobilis

Abstract

In molluscs, the shell secretion process is controlled by a set of extracellular macromolecules collectively called the shell matrix. The shell matrix, which is produced by the mantle epithelial cells during mineralization, is predominantly composed of proteins, glycoproteins, acidic polysaccharides, and chitin that precisely regulate the deposition of calcium carbonate outside the mantle cells. In the present paper, we focus on the shell of Pinna nobilis, the giant Mediterranean fan mussel, usually considered as a model for studying molluscan biomineralization processes. P. nobilis exhibits indeed a nacro-prismatic shell, the outer layer of which is constituted of the so-called “regular simple calcitic prisms,” according to Carter and Clark (1985). We review here the microstructural characteristics of the prisms and nacre and the biochemical properties of their associated matrices. In particular, the calcitic prisms of P. nobilis are characterized by a cortege of unusually acidic intraprismatic proteins, while the ones of the nacreous layer seem less acidic. A brief description of the molecular characterization of three acidic proteins, caspartin, calprismin and mucoperlin, is given. In particular, we show that extremely acidic intracrystalline proteins such as caspartin interact with calcium carbonate at different scales, from micrometric to crystal lattice levels.