Differential Effects of Two Different Routes of Immunization on Protection Against Gram-Negative Sepsis by a Detoxified Escherichia coli J5 Lipopolysaccharide Group B Meningococcal Outer Membrane Protein Complex Vaccine in a Burned Mouse Model
- 1 September 2002
- journal article
- website
- Published by Oxford University Press (OUP) in Journal of Burn Care & Rehabilitation
- Vol. 23 (5), 333-340
- https://doi.org/10.1097/00004630-200209000-00006
Abstract
Gram-negative sepsis causes morbidity and mortality in burned patients. To determine whether immunization with core endotoxin (lipopolysaccharide) via one of two routes could protect burned mice from septic death, mice were immunized either three times subcutaneously (SC) or one time intramuscularly (IM) then two times intraperitoneally (IP) with a core-lipopolysaccharide vaccine. Control mice were immunized with either saline or an irrelevant antigen. Postimmunization, mice were immunocompromised with a 15% TBSA flame burn and challenged subeschar with Klebsiella pneumoniae or Escherichia coli. Vaccine immunization improved the survival of both E. coli- and K. pneumoniae- challenged mice when given SC but not when given IM, IP. Postimmunization, total immunoglobulin titers were elevated over preimmune titers, but titers in IM, IP-immunized mice were higher than those in SC-immunized mice. Both isotyping and flow cytometry studies indicated that sera from mice immunized via IM, IP opsonized better than sera from mice immunized via SC. Hence, this vaccine provided route-specific protection of burned mice against gram-negative sepsis; its mechanism of action was not solely dependent upon increased immunoglobulin titers or phagocytosis.Keywords
This publication has 22 references indexed in Scilit:
- Escherichia coli: Epidemiology and Analysis of Risk Factors for Infections Caused by Resistant StrainsClinical Infectious Diseases, 1999
- Disseminated Intravascular CoagulationThe New England Journal of Medicine, 1999
- Bacteriology of burnsBurns, 1998
- CorrespondenceInnate Immunity, 1997
- Review: Evidence against the hypothesis that antibodies to the inner core of lipopolysaccharides in antisera raised by immunization with enterobacterial deep-rough mutants confer broad-spectrum protection during Gram-negative bacterial sepsisInnate Immunity, 1997
- A murine model with aspects of clinical relevance for the study of antibiotic-induced endotoxin release in septic hostsInnate Immunity, 1996
- A Noncovalent Complex Vaccine Prepared with Detoxified Escherichia coli J5 (Rc Chemotype) Lipopolysaccharide and Neisseria meningitidis Group B Outer Membrane Protein Produces Protective Antibodies against Gram-Negative BacteremiaThe Journal of Infectious Diseases, 1996
- Minireview: Therapeutic intervention in sepsis with antibody to endotoxin: is there a future?Innate Immunity, 1994
- Regulation of antibody isotype secretion by subsets of antigen-specific helper T cellsNature, 1988
- Features of Cross Protection Between Sindbis and Venezuelan Equine Encephalitis Viruses in Mice--Relationship of Route of Immunization to ProtectionJournal of General Virology, 1974