Small RNA class transition from siRNA/piRNA to miRNA during pre-implantation mouse development
Open Access
- 12 April 2010
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 38 (15), 5141-5151
- https://doi.org/10.1093/nar/gkq229
Abstract
Recent studies showed that small interfering RNAs (siRNAs) and Piwi-interacting RNA (piRNA) in mammalian germ cells play important roles in retrotransposon silencing and gametogenesis. However, subsequent contribution of those small RNAs to early mammalian development remains poorly understood. We investigated the expression profiles of small RNAs in mouse metaphase II oocytes, 8–16-cell stage embryos, blastocysts and the pluripotent inner cell mass (ICM) using high-throughput pyrosequencing. Here, we show that during pre-implantation development a major small RNA class changes from retrotransposon-derived small RNAs containing siRNAs and piRNAs to zygotically synthesized microRNAs (miRNAs). Some siRNAs and piRNAs are transiently upregulated and directed against specific retrotransposon classes. We also identified miRNAs expression profiles characteristic of the ICM and trophectoderm (TE) cells. Taken together, our current study reveals a major reprogramming of functional small RNAs during early mouse development from oocyte to blastocyst.Keywords
This publication has 48 references indexed in Scilit:
- MicroRNA-145 Regulates OCT4, SOX2, and KLF4 and Represses Pluripotency in Human Embryonic Stem CellsCell, 2009
- Target Protectors Reveal Dampening and Balancing of Nodal Agonist and Antagonist by miR-430Science, 2007
- MicroRNA control of Nodal signallingNature, 2007
- Characterization of the piRNA Complex from Rat TestesScience, 2006
- Identification and characterization of two novel classes of small RNAs in the mouse germline: retrotransposon-derived siRNAs in oocytes and germline small RNAs in testesGenes & Development, 2006
- A germline-specific class of small RNAs binds mammalian Piwi proteinsNature, 2006
- A novel class of small RNAs bind to MILI protein in mouse testesNature, 2006
- Zebrafish MiR-430 Promotes Deadenylation and Clearance of Maternal mRNAsScience, 2006
- MicroRNAs: Genomics, Biogenesis, Mechanism, and FunctionCell, 2004
- Role for a bidentate ribonuclease in the initiation step of RNA interferenceNature, 2001