Results of a pilot study using self‐collected mid‐turbinate nasal swabs for detection of influenza virus infection among pregnant women
Open Access
- 23 April 2015
- journal article
- Published by Wiley in Influenza and Other Respiratory Viruses
- Vol. 9 (3), 155-160
- https://doi.org/10.1111/irv.12309
Abstract
We evaluated the feasibility of asking pregnant women to self-collect and ship respiratory specimens. In a preliminary laboratory study, we compared the RT-PCR cycle threshold (CT) values of influenza A and B viruses incubated at 4 storage temperatures (from 4 to 35°C) for 6 time periods (8, 24, 48, 72, and 168 hours and 30 days), resulting in 24 conditions that were compared to an aliquot tested after standard freezing (−20°C) (baseline condition). In a subsequent pilot study, during January–February, 2014, we delivered respiratory specimen collection kits to 53 pregnant women with a medically attended acute respiratory illness using three delivery methods. CT values were stable after storage at temperatures <27°C for up to 72 hours for influenza A viruses and 48 hours for influenza B viruses. Of 53 women who received kits during the pilot, 89% collected and shipped nasal swabs as requested. However, 30% (14/47) of the women took over 2 days to collect and ship their specimen. The human control gene, ribonuclease P (RNase P), was detected in 100% of nasal swab specimens. However, the mean CT values for RNase P (26·5, 95% confidence interval [CI] = 26·0–27·1) and for the 8 influenza A virus positives in our pilot (32·2, 95% CI = 28·9–35·5) were significantly higher than the CTs observed in our 2010–2012 study using staff-collected nasal pharyngeal swabs (P-values < 0·01). Self-collection of respiratory specimens is a promising research method, but further research is needed to quantify the sensitivity and specificity of the approach.Keywords
Funding Information
- Centers for Disease Control and Prevention (200-2010-F-33132)
This publication has 25 references indexed in Scilit:
- Severity of influenza and noninfluenza acute respiratory illness among pregnant women, 2010–2012American Journal of Obstetrics and Gynecology, 2014
- Frequency of Acute Respiratory Illnesses and Circulation of Respiratory Viruses in Households With Children Over 3 Surveillance SeasonsThe Journal of Infectious Diseases, 2014
- Effectiveness of Seasonal Trivalent Influenza Vaccine for Preventing Influenza Virus Illness Among Pregnant Women: A Population-Based Case-Control Study During the 2010–2011 and 2011–2012 Influenza SeasonsClinical Infectious Diseases, 2013
- Equivalence of Self- and Staff-Collected Nasal Swabs for the Detection of Viral Respiratory PathogensPLOS ONE, 2012
- Optimal Design of Intervention Studies to Prevent Influenza in Healthy CohortsPLOS ONE, 2012
- E-mail-based symptomatic surveillance combined with self-collection of nasal swabs: a new tool for acute respiratory infection epidemiologyInternational Journal of Infectious Diseases, 2011
- Self-Collected Mid-Turbinate Swabs for the Detection of Respiratory Viruses in Adults with Acute Respiratory IllnessesPLOS ONE, 2011
- Self-collected nasal swabs to detect infection and colonization: a useful tool for population-based epidemiological studies?International Journal of Infectious Diseases, 2011
- Development and Evaluation of a Flocked Nasal Midturbinate Swab for Self-Collection in Respiratory Virus Infection Diagnostic TestingJournal of Clinical Microbiology, 2010
- Linking syndromic surveillance with virological self-samplingEpidemiology and Infection, 2007