Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor
Top Cited Papers
- 15 April 2020
- journal article
- research article
- Published by American Chemical Society (ACS) in ACS Nano
- Vol. 14 (4), 5135-5142
- https://doi.org/10.1021/acsnano.0c02823
Abstract
Coronavirus disease 2019 (COVID-19) is a newly emerging human infectious disease caused by acute respiratory syndrome coronavirus 2 (SARS-CoV-2, previously called 2019-nCoV). Based on the rapid increase in the rate of human infection, the World Health Organization (WHO) has classified the COVID-19 outbreak as a pandemic. Because no specific drugs or vaccines for COVID-19 are yet available, early diagnosis and management are crucial for containing the outbreak. Here, we report a field-effect transistor (FET)-based biosensing device for detecting SARS-CoV-2 in clinical samples. The sensor was produced by coating graphene sheets of FET with a specific antibody against SARS-CoV-2 spike protein. The performance of the sensor was determined using antigen protein, cultured virus, and nasopharyngeal swab specimens from COVID-19 patients. Our FET device could detect SARS-CoV-2 spike protein at concentrations of 1 fg/ml in PBS and 100 fg/ml clinical transport medium. In addition, the FET sensor successfully detected SARS-CoV-2 in culture medium (limit of detection [LOD]: 1.6 x 101 pfu/ml) and clinical samples (LOD: 2.42 x 102 copies/ml). Thus, we have successfully fabricated a promising FET biosensor for SARS-CoV-2; our device is a highly sensitive immunological diagnostic method for COVID-19 that requires no sample pretreatment or labeling.Keywords
Funding Information
- Ministry of Science, ICT and Future Planning (CRC-16-01-KRICT)
- Ministry of Health and Welfare, Republic of Korea (HI20C0033, HI20C363)
This publication has 24 references indexed in Scilit:
- Doping effects of surface functionalization on graphene with aromatic molecule and organic solventsApplied Surface Science, 2017
- InP Nanowire Biosensor with Tailored Biofunctionalization: Ultrasensitive and Highly Selective Disease Biomarker DetectionNano Letters, 2017
- Detection of heart failure-related biomarker in whole blood with graphene field effect transistor biosensorBiosensors and Bioelectronics, 2017
- Label-free graphene biosensor targeting cancer molecules based on non-covalent modificationBiosensors and Bioelectronics, 2017
- SARS and MERS: recent insights into emerging coronavirusesNature Reviews Microbiology, 2016
- Low‐Voltage Complementary Electronics from Ion‐Gel‐Gated Vertical Van der Waals HeterostructuresAdvanced Materials, 2016
- Giant enhancement in vertical conductivity of stacked CVD graphene sheets by self-assembled molecular layersNature Communications, 2014
- Experimental Review of GrapheneISRN Condensed Matter Physics, 2012
- The rise of grapheneNature Materials, 2007
- Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirusNature, 2003