Expanding the Versatility of Phage Display I: Efficient Display of Peptide-Tags on Protein VII of the Filamentous Phage
Open Access
- 24 February 2011
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 6 (2), e14702
- https://doi.org/10.1371/journal.pone.0014702
Abstract
Phage display is a platform for selection of specific binding molecules and this is a clear-cut motivation for increasing its performance. Polypeptides are normally displayed as fusions to the major coat protein VIII (pVIII), or the minor coat protein III (pIII). Display on other coat proteins such as pVII allows for display of heterologous peptide sequences on the virions in addition to those displayed on pIII and pVIII. In addition, pVII display is an alternative to pIII or pVIII display. Here we demonstrate how standard pIII or pVIII display phagemids are complemented with a helper phage which supports production of virions that are tagged with octa FLAG, HIS6 or AviTag on pVII. The periplasmic signal sequence required for pIII and pVIII display, and which has been added to pVII in earlier studies, is omitted altogether. Tagging on pVII is an important and very useful add-on feature to standard pIII and pVII display. Any phagemid bearing a protein of interest on either pIII or pVIII can be tagged with any of the tags depending simply on choice of helper phage. We show in this paper how such tags may be utilized for immobilization and separation as well as purification and detection of monoclonal and polyclonal phage populations.Keywords
This publication has 30 references indexed in Scilit:
- Progress in phage display: evolution of the technique and its applicationsCellular and Molecular Life Sciences, 2009
- Fabricating Genetically Engineered High-Power Lithium-Ion Batteries Using Multiple Virus GenesScience, 2009
- Design and Validation of a Bifunctional Ligand Display System for Receptor TargetingCell Chemical Biology, 2004
- Selection and Characterization of Cyclic Peptides that Bind to a Monoclonal Antibody Against Meningococcal L3,7,9 lipopolysaccharidesScandinavian Journal of Immunology, 2004
- Phage display of peptide epitopes from HIV-1 elicits strong cytolytic responsesNature Biotechnology, 2000
- [21] Phage display for selection of novel binding peptidesMethods in Enzymology, 2000
- A stimulatory monoclonal antibody detecting T cell receptor diversity among idiotype‐specific, major histocompatibility complex‐restricted T cell clonesEuropean Journal of Immunology, 1990
- Searching for Peptide Ligands with an Epitope LibraryScience, 1990
- Translation of phage f1 gene VII occurs from an inherently defective initiation site made functional by couplingJournal of Molecular Biology, 1989
- Genes VI, VII, and IX of phage M13 code for minor capsid proteins of the virion.Proceedings of the National Academy of Sciences of the United States of America, 1981