THE INFLUENCE OF PHAGOCYTOSIS ON THE INTRACELLULAR DISTRIBUTION OF GRANULE-ASSOCIATED COMPONENTS OF POLYMORPHONUCLEAR LEUCOCYTES

Abstract

Control and phagocyting populations of rabbit polymorphonuclear leucocytes have been compared in terms of the content and distribution of phagocytin and selected hydrolytic enzymes. Following incubation at 37°C., the cells were disrupted by homogenization and separated into an 8,200 g pellet and supernatant fluid. The high speed pellet from control leucocytes contained all the larger particulate elements of the cell including intact cytoplasmic granules.