Effects of Cortisol on Serially Propogated Fibroblast Cell Cultures Derived from the Rabbit Fetal Lung and Skin

Abstract

Cortisol affects the growth of serially propogated, fibroblast cell cultures derived from the rabbit fetal lung in a manner which is dependent upon the gestational age of the material used: early in gestation (20 days), the hormone (10⁻⁷–10⁻⁵ M) stimulates [6-³H]thymidine incorporation into DNA, while in late gestation (28 days), cortisol (10⁻⁷ and 10⁻⁶ M) inhibits this process. Cultures derived from the rabbit fetal skin are inhibited by cortisol (10⁻⁵ M) at both gestational ages. Fibroblasts derived from lung, but not from skin, efficiently convert cortisone to cortisol and this activity increases with advancing gestation. Cortisol does not affect the incorporation of [³H]choline into lecithin by confluent cultures of any of the fibroblast types studied.