Bacterial ApbC Can Bind and Effectively Transfer Iron−Sulfur Clusters
- 11 July 2008
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 47 (31), 8195-8202
- https://doi.org/10.1021/bi800551y
Abstract
The metabolism of iron−sulfur ([Fe-S]) clusters requires a complex set of machinery that is still being defined. Mutants of Salmonella enterica lacking apbC have nutritional and biochemical properties indicative of defects in [Fe-S] cluster metabolism. ApbC is a 40.8 kDa homodimeric ATPase and as purified contains little iron and no acid-labile sulfide. An [Fe-S] cluster was reconstituted on ApbC, generating a protein that bound 2 mol of Fe and 2 mol of S2− per ApbC monomer and had a UV−visible absorption spectrum similar to known [4Fe-4S] cluster proteins. Holo-ApbC could rapidly and effectively activate Saccharomyces cerevisiae apo-isopropylmalate isolomerase (Leu1) in vitro, a process known to require the transfer of a [4Fe-4S] cluster. Maximum activation was achieved with 2 mol of ApbC per 1 mol of apo-Leu1. This article describes the first biochemical activity of ApbC in the context of [Fe-S] cluster metabolism. The data herein support a model in which ApbC coordinates an [4Fe-4S] cluster across its dimer interface and can transfer this cluster to an apoprotein acting as an [Fe-S] cluster scaffold protein, a function recently deduced for its eukaryotic homologues.Keywords
This publication has 46 references indexed in Scilit:
- Salmonella enterica Requires ApbC Function for Growth on Tricarballylate: Evidence of Functional Redundancy between ApbC and IscUJournal of Bacteriology, 2008
- The iron–sulphur protein Ind1 is required for effective complex I assemblyThe EMBO Journal, 2008
- In Vitro Activation of Apo-Aconitase Using a [4Fe-4S] Cluster-Loaded Form of the IscU [Fe−S] Cluster Scaffolding ProteinBiochemistry, 2007
- HscA and HscB Stimulate [2Fe-2S] Cluster Transfer from IscU to Apoferredoxin in an ATP-Dependent ReactionBiochemistry, 2006
- NifS-Mediated Assembly of [4Fe−4S] Clusters in the N- and C-Terminal Domains of the NifU Scaffold ProteinBiochemistry, 2005
- Iron-Sulfur Cluster AssemblyPublished by Elsevier BV ,2004
- SufA from Erwinia chrysanthemiJournal of Biological Chemistry, 2003
- Characterization of an Iron−Sulfur Cluster Assembly Protein (ISU1) from Schizosaccharomyces pombeBiochemistry, 2002
- Kinetic Characterization of the ATPase Cycle of the Molecular Chaperone Hsc66 from Escherichia coliPublished by Elsevier BV ,2000
- IscS Is a Sulfurtransferase for the in Vitro Biosynthesis of 4-Thiouridine in Escherichia coli tRNABiochemistry, 1999