Simultaneous Catabolite Repression between Glucose and Toluene Metabolism in Pseudomonas putida Is Channeled through Different Signaling Pathways
Open Access
- 15 September 2007
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 189 (18), 6602-6610
- https://doi.org/10.1128/jb.00679-07
Abstract
Pseudomonas putida KT2440(pWW0) can use toluene via the TOL plasmid-encoded catabolic pathways and can use glucose via a series of three peripheral chromosome-encoded routes that convert glucose into 6-phosphogluconate (6PG), namely, the glucokinase pathway, in which glucose is transformed to 6PG through the action of glucokinase and glucose-6-phosphate dehydrogenase. Alternatively, glucose can be oxidized to gluconate, which can be phosphorylated by gluconokinase to 6PG or oxidized to 2-ketogluconate, which, in turn, is converted into 6PG. Our results show that KT2440 metabolizes glucose and toluene simultaneously, as revealed by net flux analysis of [ 13 C]glucose. Determination of glucokinase and gluconokinase activities in glucose metabolism, gene expression assays using a fusion of the promoter of the Pu TOL upper pathway to ′ lacZ , and global transcriptomic assays revealed simultaneous catabolite repression in the use of these two carbon sources. The effect of toluene on glucose metabolism was directed to the glucokinase branch and did not affect gluconate metabolism. Catabolite repression of the glucokinase pathway and the TOL pathway was triggered by two different catabolite repression systems. Expression from Pu was repressed mainly via PtsN in response to high levels of 2-dehydro-3-deoxygluconate-6-phosphate, whereas repression of the glucokinase pathway was channeled through Crc.Keywords
This publication has 47 references indexed in Scilit:
- Convergent Peripheral Pathways Catalyze Initial Glucose Catabolism inPseudomonas putida: Genomic and Flux AnalysisJournal of Bacteriology, 2007
- Role of the ptsN Gene Product in Catabolite Repression of the Pseudomonas putida TOL Toluene Degradation Pathway in Chemostat Cultures ▿Applied and Environmental Microbiology, 2006
- Growth phase‐dependent expression of the Pseudomonas putida KT2440 transcriptional machinery analysed with a genome‐wide DNA microarrayEnvironmental Microbiology, 2005
- Integration of Signals through Crc and PtsN in Catabolite Repression of Pseudomonas putida TOL Plasmid pWW0Applied and Environmental Microbiology, 2005
- Genetic Evidence that Catabolites of the Entner-Doudoroff Pathway Signal C Source Repression of the σ54PuPromoter ofPseudomonas putidaJournal of Bacteriology, 2004
- Inactivation of Cytochrome o Ubiquinol Oxidase Relieves Catabolic Repression of the Pseudomonas putida GPo1 Alkane Degradation PathwayJournal of Bacteriology, 2002
- The cyo operon of Pseudomonas putida is involved in carbon catabolite repression of phenol degradationMolecular Genetics and Genomics, 2001
- Isolation and phenotypic characterization ofPseudomonas aeruginosapseudorevertants containing suppressors of the catabolite repression control-defectivecrc-10alleleFEMS Microbiology Letters, 2001
- GC‐MS Analysis of Amino Acids Rapidly Provides Rich Information for Isotopomer BalancingBiotechnology Progress, 2000
- Mechanism responsible for glucose–lactose diauxie in Escherichia coli: challenge to the cAMP modelGenes to Cells, 1996