The Genome Sequence of an Isolate of Indian Citrus Ringspot Virus Infecting the Sweet Orange in India
- 15 November 2012
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 86 (22), 12446-12447
- https://doi.org/10.1128/jvi.02084-12
Abstract
Whole-genome sequencing of an isolate of Mandarivirus infecting the sweet orange [ Citrus sinensis (L) Blanco] in the western part of India (Pune) was done. The single-stranded positive-sense RNA genome of Indian citrus ringspot virus (ICRSV) Pune has 7,560 nucleotides (nt), excluding a poly(A) tail, comprised of 27.98% (2,115 nt) A, 32.12% (2,428 nt) C, 19.68% (1,488 nt) G, and 20.22% (1,529 nt) T residues. The genome, organized into six open reading frames (ORFs), shares 97.7% sequence identity with the complete genome of the ICRSV K1 isolate (AF406744.1) infecting the kinnow ( Citrus reticulate Blanco, a hybrid between King and Willow mandarins) in north India. The ICRSV Pune genome formed a complex secondary structure with a large number of unpaired cytosine-rich regions, and recombination analysis highlighted potential recombination in the ICRSV genome.Keywords
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