Ultrasensitive Electrochemical Detection of Prostate-Specific Antigen by Using Antibodies Anchored on a DNA Nanostructural Scaffold
- 26 June 2014
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 86 (15), 7337-7342
- https://doi.org/10.1021/ac500054x
Abstract
The high occurrence of prostate cancer in men makes the prostate-specific antigen (PSA) screening test really important. More importantly, the recurrence rate after radical prostatectomy is high, whereas the traditional PSA immunoassay does not possess the sufficient high sensitivity for post-treatment PSA detection. In these assays, uncontrolled and random orientation of capture antibodies on the surface largely reduces their activity. Here, by exploiting the rapidly emerging DNA nanotechnology, we developed a DNA nanostructure based scaffold to precisely control the assembly of antibody monolayer. We demonstrated that the detection sensitivity was critically dependent on the nanoscale-spacing (nanospacing) of immobilized antibodies. In addition to the controlled assembly, we further amplified the sensing signal by using the gold nanoparticles, resulting in extremely high sensitivity and a low detection limit of 1 pg/mL. To test the real-world applicability of our nanoengineered electrochemical sensor, we evaluated the performance with 11 patients’ serum samples and obtained consistent results with the “gold-standard” assays.Keywords
This publication has 28 references indexed in Scilit:
- Magnetic Bead-Based Reverse Colorimetric Immunoassay Strategy for Sensing BiomoleculesAnalytical Chemistry, 2013
- Sub-nanomolar Detection of Prostate-Specific Membrane Antigen in Synthetic Urine by Synergistic, Dual-Ligand PhageJournal of the American Chemical Society, 2013
- Oriented Surface Immobilization of Antibodies at the Conserved Nucleotide Binding Site for Enhanced Antigen DetectionLangmuir, 2012
- Multiple recognition assay reveals prostasomes as promising plasma biomarkers for prostate cancer.Expert Review of Anticancer Therapy, 2011
- Multiple recognition assay reveals prostasomes as promising plasma biomarkers for prostate cancerProceedings of the National Academy of Sciences of the United States of America, 2011
- Direct Observation of Adsorption-Induced Inactivation of Antibody Fragments Surrounded by Mixed-PEG Layer on a Gold SurfaceJournal of the American Chemical Society, 2010
- Nanoparticle-based bio-barcode assay redefines “undetectable” PSA and biochemical recurrence after radical prostatectomyProceedings of the National Academy of Sciences of the United States of America, 2009
- Making it stick: convection, reaction and diffusion in surface-based biosensorsNature Biotechnology, 2008
- How Sensitive Is a Prostate-Specific Antigen Measurement?: How Sensitive Does It Need to Be?Archives of Pathology & Laboratory Medicine, 2004
- Nanoparticle-Based Bio-Bar Codes for the Ultrasensitive Detection of ProteinsScience, 2003